View & Download CD data for CD0006468000

Tumor Antigen P53 N-terminal Transactivation Domain (1-73) (p53-tad)


Citation: Gabor Nagy, Soren V Hoffman, Nykola C Jones, Helmut Grubmuller 2023 Applied Spectroscopy 78/9 897-911


Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)


CSA/ACS Standard Spectrum Millidegrees_(theta) Download
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Raw Sample Spectra Millidegrees_(theta) Download
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Raw Sample Spectra 2 Millidegrees_(theta) Download
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Raw Sample Spectra 3 Millidegrees_(theta) Download
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Raw Baseline Spectra Millidegrees_(theta) Download
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Raw Baseline Spectra 2 Millidegrees_(theta) Download
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Raw Baseline Spectra 3 Millidegrees_(theta) Download
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Average Sample Millidegrees_(theta) Download
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Averaged Baseline Millidegrees_(theta) Download
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HT / High Voltage / Dynode Spectra HT/Dynode Units Download
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Net Smoothed Spectrum Millidegrees_(theta) Download
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Final Processed Spectrum Delta_Epsilon Download
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Validation report compiled by Validichro v1.4.0.1, 2024-10-16 13:08:07. - FLAG

At a glance
Downloads 256
Depositor Gabor Nagy
Uniprot P04637
Alpha Fold
PDB
EC
CATH Class
Protein Type intrinsically_disordered

Sample

Protein Name Tumor antigen P53 N-terminal Transactivation domain (1-73) ?
Alternative Protein Names P53-TAD ?
Source Organism Human (Homo Sapiens) ?
Protein Supplier Karebay Biochem Inc. ?
Expression System or natural source Other- custom peptide synthesis ?
Expressed As Mutant ?
Mutation Details P72R ?
Expression tags (if any) GS ?
Ligands Present and Concentration or ratio No data provided ?
Macromolecular Partner(s) and Concentration or ratio No data provided ?
Deposition Date 2023-11-03 ?

Experiment

CD or SRCD SRCD ?
Protein Concentration (mg/ml) 0.478 ?
Concentration Quantification Method Extinction Coefficient (214 nm + 280 nm) ?
Protein Purity (%) 95 ?
Purity Quantification Method No data provided ?
Buffer Contents and Concentrations Na2HPO4 buffer 10 mM, NaF 50 mM ?
Baseline Contents Na2HPO4 buffer 10 mM, NaF 50 mM ?
Experimental Temperature (C) 25 ?
Instrument or beamline AU-CD beamline, ISA-ASTRID2 ?
Detector Angle (Scattering Angle) No data provided ?
Sample Cell Pathlength (cm) 0.01023 ?
Cell Pathlength Calibration Method Dilution Standards ?
Sample Cell Type Cylindrical-Demountable ?
Sample Cell Composition Quartz ?
Sample Chamber Atmosphere Nitrogen ?
Number of repeat scans 3 ?
Continuous or Stepped scan Continuous ?
Maximum (highest) wavelength, nm 280 ?
Minimum (lowest) wavelength, nm 178 ?
Criteria for low wavelength cutoff HT value ?
Wavelength interval, nm 1 ?
Dwell or Averaging time, seconds No data provided ?
Experimental Collection date 2017-06-06 ?
Local Spectrum Identifier 56276 ?

Calibration

CSA or ACS CSA ?
Dichroism Units for CSA Standard Millidegrees_(theta) ?
Final Spectrum Calibrated NO ?
CSA/ACS Standard Concentration (mg/ml) 7.4 ?
CSA/ACS Pathlength (mm) 1 ?
CSA/ACS Zeroed at No data provided ?
CSA/ACS Date Measured 2017-06-06 ?
CSA/ACS Ratio (192.5nm and 290.0 nm) 2.06 ?
CD signal at 290nm (mdeg) 25.5714 ?
CSA/ACS Experiment temperature, C 25 ?

Data process.

Molecular Weight 8349.9 ?
Number of Residues 75 ?
Mean Residue Weight 112.8 ?
Data Processing Software Name Spectra Manager, SESCA ?
Data Processing Software Version No data provided ?
Wavelength Range for Zeroing No data provided ?
Number of Smoothing Points 7 ?

Sec. struct.

Secondary Structure Calculated from No data provided ?
DSSP value: alpha helix No data provided ?
DSSP value: 3-10 helix No data provided ?
DSSP value: pi helix No data provided ?
DSSP value: beta strand No data provided ?
DSSP value: beta bridge No data provided ?
DSSP value: bonded turn No data provided ?
DSSP value: bend No data provided ?
DSSP value: loop or irregular No data provided ?

Tertiary

PDB ID No data provided ?
UniProt ID P04637 ?
Enzyme Classification (EC) No data provided ?
Medline Entry No data provided ?
Cath Classification No data provided ?
Sequence GSMEEPQSDP SVEPPLSQET FSDLWKLLPE NNVLSPLPSQ AMDDLMLSPD DIEQWFTEDP GPDEAPRMPE AAPRV ?
Type of protein intrinsically_disordered ?
Keyword/phrase A P53 ?
Keyword/phrase B P53-TAD ?
Keyword/phrase C IDP ?
Keyword/phrase D IDP8 ?
Keyword/phrase E No data provided ?
Keyword/phrase F No data provided ?
Keyword/phrase G No data provided ?
Keyword/phrase H No data provided ?
Keyword/phrase I No data provided ?
Keyword/phrase J No data provided ?
Publication Authors Gabor Nagy, Soren V Hoffman, Nykola C Jones, Helmut Grubmuller ?
Publication Year 2023 ?
Publication Journal Applied Spectroscopy ?
Publication Title A Reference Data Set for Circular Dichroism Spectroscopy Comprised of Validated Intrinsically Disordered Protein Models ?
Publication Volume 78/9 ?
Publication Pages 897-911 ?

Depositor

Depositor Name Gabor Nagy ?
Department/School name Theoreritical and Computational Biophysics ?
University/Institution/Corporation Max Planck Institute for Multidisciplinary Sciences ?
Depositor Country Germany ?
Name of Principal Investigator (if not depositor) Gabor Nagy ?

Validation report compiled by Validichro v1.4.0.1, 2024-10-16 13:08:07. - FLAG

Depositors Notes:

Missing Wavelengths PASS ?
Maximum Delta Epsilon PASS ?
Minimal Level of Maximum Delta Epsilon PASS ?
Peak Locations PASS ?
Feature Width PASS ?
Experimental Temperature PASS ?
UniProt sequence ?
Molecular Weight PASS ?
Number of Residues PASS ?
Mean Residue Weight value PASS ?
Concentration and Pathlength PASS ?
CSA / ACS peak ratio PASS ?
CSA / ACS Temperature PASS ?
Peak Shift test FLAG ?
Standard Deviation PASS ?
Noise: 260-270nm PASS ?
Flat topped peaks PASS ?
Wavelength range PASS ?
Interval resolution PASS ?
High Tension Voltage 240-260nm PASS ?
Projection Test PASS ?
Standard Deviation At Peak PASS ?
Depositor Note ?

The PCDDB is a development of the Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the schools and the authors.