View & Download CD data for CD0006464001
Alpha-Synuclein
Citation: Gabor Nagy, Soren V Hoffman, Nykola C Jones, Helmut Grubmuller 2024 Applied Spectroscopy 78/9 897-911
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | Download |
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| Raw Sample Spectra | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 4 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 5 | Millidegrees_(theta) | Download |
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| Average Sample | Millidegrees_(theta) | Download |
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| Averaged Baseline | Millidegrees_(theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees_(theta) | Download |
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| Final Processed Spectrum | Delta_Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2025-11-12 11:25:41. - FLAG
At a glance
| Downloads | 132 |
| Depositor | Gabor Nagy |
| Uniprot |
P37840 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | intrinsically_disordered |
Sample
| Protein Name | Alpha-Synuclein | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Human | ? |
| Protein Supplier | Max Planck Institute for Biophysical Chemistry | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2024-05-23 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 1.07 | ? |
| Concentration Quantification Method | Extinction Coefficient | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | Sodium Phosphate 10 mM, Sodium Fluoride 50 mM | ? |
| Baseline Contents | Sodium Phosphate 10 mM, Sodium Fluoride 50 mM | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | AU-CD beamline, ASTRID2, Aarhus, Denmark | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01023 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Closed | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 5 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 178 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2016-11-23 | ? |
| Local Spectrum Identifier | 52744 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | NO | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.4 | ? |
| CSA/ACS Pathlength (mm) | 11 | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 2024-05-23 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.06 | ? |
| CD signal at 290nm (mdeg) | 24.893 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P37840 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA | ? |
| Type of protein | intrinsically_disordered | ? |
| Keyword/phrase A | Alpha-Synuclein | ? |
| Keyword/phrase B | IDP | ? |
| Keyword/phrase C | IDP8 | ? |
| Keyword/phrase D | Intrisically Disordered Protein | ? |
| Keyword/phrase E | Asyn | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Gabor Nagy, Soren V Hoffman, Nykola C Jones, Helmut Grubmuller | ? |
| Publication Year | 2024 | ? |
| Publication Journal | Applied Spectroscopy | ? |
| Publication Title | Reference Data Set for Circular Dichroism Spectroscopy Comprised of Validated Intrinsically Disordered Protein Models | ? |
| Publication Volume | 78/9 | ? |
| Publication Pages | 897-911 | ? |
Validation report compiled by Validichro v1.4.0.1, 2025-11-12 11:25:41. - FLAG
Depositors Notes: Reuploaded CD spectrum entry, with publication data
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | FLAG | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Reuploaded CD spectrum entry, with publication data | ? |