View & Download CD data for CD0006463000
Measles Virus Nucleocapsid Tail (401-525) (mev-ntail)
Citation: Francesca Trolio, Daniela Bonetti, Christophe Bignon, Sonia Longhi, Stefano Gianni 2019 ACS Chemical Biology 14 337-341
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | File not provided | |
| Raw Sample Spectra | File not provided | ||
| Raw Baseline Spectra | File not provided | ||
| Average Sample | File not provided | ||
| Averaged Baseline | File not provided | ||
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | File not provided | ||
| Final Processed Spectrum | Delta_Epsilon | Download |
View Plot |
Validation report compiled by Validichro v1.4.0.1, 2023-11-07 14:15:10. - PASS (incomplete)
| Downloads | 470 |
| Depositor | Gabor Nagy |
| Uniprot |
Q89933 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | intrinsically_disordered |
Sample
| Protein Name | Measles Virus Nucleocapsid Tail (401-525) | ? |
| Alternative Protein Names | MeV-Ntail | ? |
| Source Organism | E. Coli | ? |
| Protein Supplier | Aix Marseille University, CNRS, AFMB, Marseille, France | ? |
| Expression System or natural source | E. Coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | His6-tag, TEV restriction site | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2023-10-23 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.1 | ? |
| Concentration Quantification Method | Extinction Coefficient | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | Na2HPO4 buffer 10 mM, NaCl 150 mM | ? |
| Baseline Contents | Na2HPO4 buffer 10 mM, NaCl 150 mM | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | JASCO-810 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | Dilution Standards | ? |
| Sample Cell Type | Rectangular-Demountable | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 190 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2018-01-08 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | NO | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 2023-11-06 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | Q89933 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | GSTTEDKISR AVGPRQAQVS FLHGDQSENE LPRLGGKEDR RVKQSRGEAR ESYRETGPSR ASDARAAHLP TGTPLDIDTA SESSQDPQDS RRSADALLRL QAMAGISEEQ GSDTDTPIVY NDRNLLD | ? |
| Type of protein | intrinsically_disordered | ? |
| Keyword/phrase A | MeV-Ntail | ? |
| Keyword/phrase B | Ntail | ? |
| Keyword/phrase C | MeV | ? |
| Keyword/phrase D | Nucleocapsid | ? |
| Keyword/phrase E | Measles | ? |
| Keyword/phrase F | Virus | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Francesca Trolio, Daniela Bonetti, Christophe Bignon, Sonia Longhi, Stefano Gianni | ? |
| Publication Year | 2019 | ? |
| Publication Journal | ACS Chemical Biology | ? |
| Publication Title | Understanding Intramolecular crosstalk in an Intrinsically Disordered Protein | ? |
| Publication Volume | 14 | ? |
| Publication Pages | 337-341 | ? |
Validation report compiled by Validichro v1.4.0.1, 2023-11-07 14:15:10. - PASS (incomplete)
Depositors Notes: This CD spectrum was provided to the depositor by Prof. Sonia Longhi (the owner of the spectrum). Permission to deposit the spectrum was granted before deposition.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | This CD spectrum was provided to the depositor by Prof. Sonia Longhi (the owner of the spectrum). Permission to deposit the spectrum was granted before deposition. | ? |