View & Download CD data for CD0006461003
HNF-1A DBD-TAD 50% TFE
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | File not provided | |
| Raw Sample Spectra | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees_(theta) | Download |
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| Average Sample | Download |
File not provided | |
| Averaged Baseline | Download |
File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
File not provided |
| Net Smoothed Spectrum | Download |
File not provided | |
| Final Processed Spectrum | Delta_Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2023-09-08 11:56:07. - PASS (incomplete)
At a glance
| Downloads | 909 |
| Depositor | Laura Kind |
| Uniprot |
P20823 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | intrinsically_disordered |
Sample
| Protein Name | HNF-1A DBD-TAD 50% TFE | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Homo sapiens | ? |
| Protein Supplier | Thomas Arnesen lab, University of Bergen, Department of Biomedicine, Bergen, Norway | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Other-Specify | ? |
| Mutation Details | residues 83 - 631 | ? |
| Expression tags (if any) | N, MGPHHHHHHLESTSLYKKAGSENLYFQG | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2023-08-28 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 0.5 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 20 mM sodium phosphate pH 7.8, 150 mM NaF, 1 mM TCEP, 50% TFE | ? |
| Baseline Contents | 20 mM sodium phosphate pH 7.8, 150 mM NaF, 1 mM TCEP, 50% TFE | ? |
| Experimental Temperature (C) | 10 | ? |
| Instrument or beamline | AU-CD beamline, ASTRID2 synchrotron (Aarhus, Denmark) | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2021-12-13 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | NO | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | No data provided | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P20823 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MGPHHHHHHL ESTSLYKKAG SENLYFQGPP ILKELENLSP EEAAHQKAVV ETLLQEDPWR VAKMVKSYLQ QHNIPQREVV DTTGLNQSHL SQHLNKGTPM KTQKRAALYT WYVRKQREVA QQFTHAGQGG LIEEPTGDEL PTKKGRRNRF KWGPASQQIL FQAYERQKNP SKEERETLVE ECNRAECIQR GVSPSQAQGL GSNLVTEVRV YNWFANRRKE EAFRHKLAMD TYSGPPPGPG PGPALPAHSS PGLPPPALSP SKVHGVRYGQ PATSETAEVP SSSGGPLVTV STPLHQVSPT GLEPSHSLLS TEAKLVSAAG GPLPPVSTLT ALHSLEQTSP GLNQQPQNLI MASLPGVMTI GPGEPASLGP TFTNTGASTL VIGLASTQAQ SVPVINSMGS SLTTLQPVQF SQPLHPSYQQ PLMPPVQSHV TQSPFMATMA QLQSPHALYS HKPEVAQYTH TGLLPQTMLI TDTTNLSALA SLTPTKQVFT SDTEASSESG LHTPASQATT LHVPSQDPAG IQHLQPAHRL SASPTVSSSS LVLYQSSDSS NGQSHLLPSN HSVIETFIST QMASSSQ | ? |
| Type of protein | intrinsically_disordered | ? |
| Keyword/phrase A | transcription factor | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2023-09-08 11:56:07. - PASS (incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Minimal Level of Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Peak Locations | INSUFFICIENT DATA | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | INSUFFICIENT DATA | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | INSUFFICIENT DATA | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | INSUFFICIENT DATA | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |