View & Download CD data for CD0006414000
Ribosome-inactivating Protein Saporin-6 (saporin)
Citation: Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace 2022
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | Download |
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| Raw Sample Spectra | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees_(theta) | Download |
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| Average Sample | Millidegrees_(theta) | Download |
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| Averaged Baseline | Millidegrees_(theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees_(theta) | Download |
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| Final Processed Spectrum | Delta_Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2023-07-30 12:07:16. - PASS
| Downloads | 1881 |
| Depositor | Andrew Miles |
| Uniprot |
P20656 |
| Alpha Fold | |
| PDB |
1qi7 |
| EC | |
| CATH Class | |
| Protein Type | soluble_globular |
Sample
| Protein Name | Ribosome-inactivating protein saporin-6 | ? |
| Alternative Protein Names | saporin | ? |
| Source Organism | Saponaria officinalis | ? |
| Protein Supplier | Sigma Aldrich | ? |
| Expression System or natural source | Natural source | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2022-06-21 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 4.2 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 20mM phosphate pH7.0, 10% glycerol | ? |
| Baseline Contents | 20mM phosphate pH7.0, 10% glycerol | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2017-04-29 | ? |
| Local Spectrum Identifier | a55276 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.5 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 235-245 | ? |
| CSA/ACS Date Measured | 2017-04-27 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.15 | ? |
| CD signal at 290nm (mdeg) | 25.7 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 1qi7 | ? |
| UniProt ID | P20656 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKEKF LRINFQSSRG TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAEST ALFPEATTAN QKALEYTEDY QSIEKNAQIT QGDQSRKELG LGIDLLSTSM EAVNKKARVV KDEARFLLIA IQMTAEAARF RYIQNLVIKN FPNKFNSENK VIQFEVNWKK ISTAIYGDAK NGVFNKDYDF GFGKVRQVKD LQMGLLMYLG KPK | ? |
| Type of protein | soluble_globular | ? |
| Keyword/phrase A | IDP175 test | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace | ? |
| Publication Year | 2022 | ? |
| Publication Journal | No data provided | ? |
| Publication Title | DichroIDP: A Novel Method for Analyses of Intrinsically Disordered Proteins by Circular Dichroism Spectroscopy | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2023-07-30 12:07:16. - PASS
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |