View & Download CD data for CD0006411000
Cyclin-dependent-kinase Inhibitor Sic1
Citation: Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace 2022
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | File not provided | |
| Raw Sample Spectra | File not provided | ||
| Raw Baseline Spectra | File not provided | ||
| Average Sample | File not provided | ||
| Averaged Baseline | File not provided | ||
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | File not provided | ||
| Final Processed Spectrum | Delta_Epsilon | Download |
View Plot |
Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:40:06. - FLAG (incomplete)
| Downloads | 942 |
| Depositor | Andrew Miles |
| Uniprot |
P38634 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | intrinsically_disordered |
Sample
| Protein Name | cyclin-dependent-kinase inhibitor Sic1 | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Saccharomyces cerevisiae | ? |
| Protein Supplier | digitized | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2022-06-21 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | unknown | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | 95 | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 50mM sodium phosphate pH 6.5, 300mM Nacl | ? |
| Baseline Contents | 50mM sodium phosphate pH 6.5, 300mM Nacl | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | unknown | ? |
| Sample Cell Type | unknown | ? |
| Sample Cell Composition | unknown | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 190 | ? |
| Criteria for low wavelength cutoff | unknown | ? |
| Wavelength interval, nm | 0.5 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 1970-01-01 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | NO | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 2022-06-26 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | AlphaFold | ? |
| DSSP value: alpha helix | 0.114 | ? |
| DSSP value: 3-10 helix | 0.013 | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | 0.039 | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | 0.044 | ? |
| DSSP value: bend | 0.052 | ? |
| DSSP value: loop or irregular | 0.739 | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P38634 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK SFKNAPLLAP PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG TVREEQEPLG RVNRILFPTQ QNVDIDAAEE EEEGEVLLPP SRPTSARQLH LSLERDEFDQ THRKKIIKDV PGTPSDKVIT FELAKNWNNN SPKNDARSQE SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE NPDIEDVITY VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH | ? |
| Type of protein | intrinsically_disordered | ? |
| Keyword/phrase A | IDP175 test | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace | ? |
| Publication Year | 2022 | ? |
| Publication Journal | No data provided | ? |
| Publication Title | DichroIDP: A Novel Method for Analyses of Intrinsically Disordered Proteins by Circular Dichroism Spectroscopy | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:40:06. - FLAG (incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | INSUFFICIENT DATA | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | FLAG | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |