View & Download CD data for CD0006408000
Translocated Actin Recruiting Phosphoprotein (tarp)
Citation: Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace 2022
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | Download |
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| Raw Sample Spectra | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees_(theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees_(theta) | Download |
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| Average Sample | Millidegrees_(theta) | Download |
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| Averaged Baseline | Millidegrees_(theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees_(theta) | Download |
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| Final Processed Spectrum | Delta_Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:34:20. - PASS
| Downloads | 1606 |
| Depositor | Andrew Miles |
| Uniprot |
Q6GX35 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | peptide |
Sample
| Protein Name | translocated actin recruiting phosphoprotein | ? |
| Alternative Protein Names | TARP | ? |
| Source Organism | Chlamydia trachomatis | ? |
| Protein Supplier | Tharin Blumenschein, University of East Anglia | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | 174-222 | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2022-06-21 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 7.9 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10mM tris, 100mM NaCl | ? |
| Baseline Contents | 10mM tris, 100mM NaCl | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0011 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2020-02-01 | ? |
| Local Spectrum Identifier | R73164 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.2 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 230-235 | ? |
| CSA/ACS Date Measured | 2022-06-22 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.12 | ? |
| CD signal at 290nm (mdeg) | 23.9 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | AlphaFold | ? |
| DSSP value: alpha helix | 0.041 | ? |
| DSSP value: 3-10 helix | 0.024 | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | 0.082 | ? |
| DSSP value: bend | 0.082 | ? |
| DSSP value: loop or irregular | 0.771 | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | Q6GX35 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | PTVTVSTSSS STQATATSNK TSQAVAGKIT SPDTSESSET SSTSSSDHI | ? |
| Type of protein | peptide | ? |
| Keyword/phrase A | IDP175 | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace | ? |
| Publication Year | 2022 | ? |
| Publication Journal | No data provided | ? |
| Publication Title | DichroIDP: A Novel Method for Analyses of Intrinsically Disordered Proteins by Circular Dichroism Spectroscopy | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:34:20. - PASS
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |