Hydrophilic Acylated Surface Protein A (haspa)

Citation: Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace 2022

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees_(theta)	Download	View Plot
Raw Sample Spectra	Millidegrees_(theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees_(theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra 4	Millidegrees_(theta)	Download	View Plot
Average Sample	Millidegrees_(theta)	Download	View Plot
Averaged Baseline	Millidegrees_(theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees_(theta)	Download	View Plot
Final Processed Spectrum	Delta_Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:29:39. - FLAG

At a glance

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Sample

Protein Name	hydrophilic acylated surface protein A	?
Alternative Protein Names	HASPA	?
Source Organism	Leishmania major	?
Protein Supplier	Panethymitaki, C., Imperial College London	?
Expression System or natural source	Natural source	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2022-06-21	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	5.0	?
Concentration Quantification Method	A280 or A280-denatured	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	10mM phosphate pH 7.4	?
Baseline Contents	10mM phosphate pH 7.4	?
Experimental Temperature (C)	20	?
Instrument or beamline	ISA CD1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.0015	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	300	?
Minimum (lowest) wavelength, nm	160	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	2	?
Experimental Collection date	2003-08-19	?
Local Spectrum Identifier	a3214.gen	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees_(theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	10.06	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	340-350	?
CSA/ACS Date Measured	2022-06-22	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	1.89	?
CD signal at 290nm (mdeg)	32.7	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	7562.8	?
Number of Residues	73	?
Mean Residue Weight	105	?
Data Processing Software Name	CDToolX	?
Data Processing Software Version	1.2	?
Wavelength Range for Zeroing	263-267	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	AlphaFold2	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	0.005	?
DSSP value: bend	0.038	?
DSSP value: loop or irregular	0.956	?

Tertiary

PDB ID	No data provided	?
UniProt ID	P90552	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	MGSSCTKDSA KEPQKSAGNI DTTTRSDEKD GVLVQQNDGD VQKKSEDGDN VGEGGKGNED GNDDQPKEHA AGN	?
Type of protein	intrinsically_disordered	?
Keyword/phrase A	IDP175	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	Andrew J. Miles, Elliot D. Drew, R.W. Janes, B.A. Wallace	?
Publication Year	2022	?
Publication Journal	No data provided	?
Publication Title	DichroIDP: A Novel Method for Analyses of Intrinsically Disordered Proteins by Circular Dichroism Spectroscopy	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	B. A. Wallace	?

Validation report compiled by Validichro v1.4.0.1, 2023-07-30 11:29:39. - FLAG

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	FLAG	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence		?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?