View & Download CD data for CD0006395001
BH1501 Protein T1 Chimera (nachbac bacterial voltage gated sodium channel t1 chimera)
Citation: O’Reilly A.O., Powl A.M., D’Avanzo N., Miles A.J., Nichols C.G., Wallace B.A. 2022 FEBS letters
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees_(theta) | Download |
View Plot |
| Raw Sample Spectra | Download |
File not provided | |
| Raw Baseline Spectra | Download |
File not provided | |
| Average Sample | Download |
File not provided | |
| Averaged Baseline | Download |
File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
View Plot |
| Net Smoothed Spectrum | Download |
File not provided | |
| Final Processed Spectrum | Delta_Epsilon | Download |
View Plot |
Validation report compiled by Validichro v1.4.0.1, 2022-01-01 18:27:44. - PASS (incomplete)
| Downloads | 564 |
| Depositor | Andrew Miles |
| Uniprot |
Q9KCR8 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | membrane |
Sample
| Protein Name | BH1501 protein T1 chimera | ? |
| Alternative Protein Names | NachBac Bacterial voltage gated sodium channel T1 chimera | ? |
| Source Organism | B. halodurans | ? |
| Protein Supplier | A. O. O'Reilly | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | deletion and insertion | ? |
| Mutation Details | C, termination at position 239. Addition at N of T1-tetramerisation domain of the Kv1.2 channel | ? |
| Expression tags (if any) | N,MGSSHHHHHHSSGLVPRGSH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2022-01-01 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 1.35 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | 95 | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 20 mM sodium phosphate; pH 7.6, 100 mM NaCl, % Cymal-5 | ? |
| Baseline Contents | 20 mM sodium phosphate; pH 7.6, 100 mM NaCl, 0.3 % Cymal-5 | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | Aviv | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.005 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1 | ? |
| Experimental Collection date | 2010-02-21 | ? |
| Local Spectrum Identifier | KBach #1 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees_(theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 5.9 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 235-245 | ? |
| CSA/ACS Date Measured | 2009-09-09 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.1 | ? |
| CD signal at 290nm (mdeg) | 22.8 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | Q9KCR8 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MGSSHHHHHH SSGLVPRGSH MTVATGDPAD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT LAQFPETLLG DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR PVNVPLDIFS EEIRFYELGE EAMEMFREDE GYIKEEERPL PENEFQKQNS FTSKMQKIVN HRAFTFTVIA LILFNALIVG IETYPRIYAD HKWLFYRIDL VLLWIFTIEI AMRFLASNPK SAFFRSSWNW FDFLIVAAGH IFAGAQFVTV LRILRVLRVL RAISVVPSLR RLVDALVMTI PALGNILILM SIFFYIFAVI GTMLFQHVSP EYFGNLQLSL LTLFQVVTLE SWASGVMRPI FAEVPWSWLY FVSFVLIGTF IIFNLFIGVI VNNVEKAELT | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | NaChBac voltage gated sodium channel | ? |
| Keyword/phrase B | T1 chimera | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | O’Reilly A.O., Powl A.M., D’Avanzo N., Miles A.J., Nichols C.G., Wallace B.A. | ? |
| Publication Year | 2022 | ? |
| Publication Journal | FEBS letters | ? |
| Publication Title | The T1-tetramerization Domain of Kv1.2 Rescues Expression and Preserves Function of a Truncated NaChBac Sodium Channel | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2022-01-01 18:27:44. - PASS (incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |