View & Download CD data for CD0006230000
Pyrroline-5-carboxylate Reductase 2 (pycr2, p5cr2)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | File not provided | ||
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | Millidegrees_(theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees_(theta) | Download |
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| Final Processed Spectrum | Mean Residue Ellipticity | Download |
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Validation report compiled by Validichro v1.4.0.1, 2021-03-22 21:30:28. - FLAG (incomplete)
| Downloads | 950 |
| Depositor | Sagar M. Patel |
| Uniprot |
Q96C36 |
| Alpha Fold | |
| PDB |
6LHM |
| EC | 1.5.1.2 |
| CATH Class | |
| Protein Type | soluble_globular |
Sample
| Protein Name | Pyrroline-5-carboxylate reductase 2 | ? |
| Alternative Protein Names | PYCR2, P5CR2 | ? |
| Source Organism | Homo sapiens (Human) | ? |
| Protein Supplier | Dr. Donald F. Becker (University of Nebraska-Lincoln) | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Mutant (single site) | ? |
| Mutation Details | R251C | ? |
| Expression tags (if any) | N,MDHHHHHHHHASENLYFQGH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2019-08-23 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.144299 | ? |
| Concentration Quantification Method | 660 nm Pierce Protein Dye Assay (ThermoFisher Scientific-Pierce Biotech) | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10 mM NaH2PO4, 250 mM NH4F, pH 8 | ? |
| Baseline Contents | 10 mM NaH2PO4, 250 mM NH4F, pH 8 | ? |
| Experimental Temperature (C) | 22 | ? |
| Instrument or beamline | Jasco CD spectrophotometer Model J-815 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 16 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 0.5 | ? |
| Dwell or Averaging time, seconds | 4 | ? |
| Experimental Collection date | 2018-04-13 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | No data provided | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | No data provided | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | No data provided | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | BeStSel beta-structure selection algorithm | ? |
| DSSP value: alpha helix | 0.000 | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | 0.449 | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | 0.154 | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | 6LHM | ? |
| UniProt ID | Q96C36 | ? |
| Enzyme Classification (EC) | 1.5.1.2 | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MDHHHHHHHH ASENLYFQGH MSVGFIGAGQ LAYALARGFT AAGILSAHKI IASSPEMNLP TVSALRKMGV NLTRSNKETV KHSDVLFLAV KPHIIPFILD EIGADVQARH IVVSCAAGVT ISSVEKKLMA FQPAPKVIRC MTNTPVVVQE GATVYATGTH ALVEDGQLLE QLMSSVGFCT EVEEDLIDAV TGLSGSGPAY AFMALDALAD GGVKMGLPRR LAIQLGAQAL LGAAKMLLDS EQHPCQLKDN VCSPGGATIH ALHFLESGGF CSLLINAVEA SCIRTRELQS MADQEKISPA ALKKTLLDRV KLESPTVSTL TPSSPGKLLT RSLALGGKKD | ? |
| Type of protein | soluble_globular | ? |
| Keyword/phrase A | proline biosynthesis | ? |
| Keyword/phrase B | hypomyelinating leukodystrophy type 10 with microcephaly | ? |
| Keyword/phrase C | recycling NAD(P)H redox equivalents | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2021-03-22 21:30:28. - FLAG (incomplete)
Depositors Notes: Despite multiple attempts of entering correct UniProt code (Q96C36) for Pyrroline-5-carboxylate reductase 2 (Homo sapiens) here, I kept noticing error of retrieving that UniProt code for INSUFFICIENT DATA reported. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated using the pathlength 0.01 cm, my experimental CD spectra of enzyme concentration gradients revealed 4 uM was the most feasible with my limited concentration of available protein.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | Despite multiple attempts of entering correct UniProt code (Q96C36) for Pyrroline-5-carboxylate reductase 2 (Homo sapiens) here, I kept noticing error of retrieving that UniProt code for INSUFFICIENT DATA reported. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated using the pathlength 0.01 cm, my experimental CD spectra of enzyme concentration gradients revealed 4 uM was the most feasible with my limited concentration of available protein. | ? |