View & Download CD data for CD0006224006
NavMs Full Length (prokaryote voltage-gated sodium channel)
Citation: Geancarlo Zanatta, Altin Sula, Andrew J. Miles, Dave Leo Ng , Paul G. DeCaen, B.A. Wallace 2019 Proc Natl Acad Sci (USA) https://doi.org/10.1073/pnas.1909696116
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Average Sample | ---- Please select ---- | Download |
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| Averaged Baseline | ---- Please select ---- | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2019-12-02, 11:57am. - PASS (Incomplete)
| Downloads | 789 |
| Depositor | Andrew Miles |
| Uniprot |
A0L5S6 |
| Alpha Fold | |
| PDB |
5HVD |
| EC | |
| CATH Class | |
| Protein Type | membrane |
Sample
| Protein Name | NavMs Full length | ? |
| Alternative Protein Names | Prokaryote voltage-gated sodium channel | ? |
| Source Organism | Magnetococcus marinus | ? |
| Protein Supplier | Altin Sula, Geancarlo Zanatta | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Mutant | ? |
| Mutation Details | I218C | ? |
| Expression tags (if any) | N. GSH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2019-11-21 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 0.82 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 20 mM Tris, 24 mM NaCl, 270 mM NaF, 0.2%DDM, 10% Glycerol | ? |
| Baseline Contents | 20 mM Tris, 24 mM NaCl, 270 mM NaF, 0.2%DDM, 10% Glycerol | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 270 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2015-12-10 | ? |
| Local Spectrum Identifier | a49957.gen | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.4 | ? |
| CSA/ACS Pathlength (mm) | 0.01 | ? |
| CSA/ACS Zeroed at | 235-240 | ? |
| CSA/ACS Date Measured | 2015-12-08 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.04 | ? |
| CD signal at 290nm (mdeg) | 26.45 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | 5HVD | ? |
| UniProt ID | A0L5S6 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | GSHMSRKIRD LIESKRFQNV ITAIIVLNGA VLGLLTDTTL SASSQNLLER VDQLCLTIFI VEISLKIYAY GVRGFFRSGW NLFDFVIVAI ALMPAQGSLS VLRTFRIFRV MRLVSVIPTM RRVVQGMLLA LPGVGSVAAL LTVVFYIAAV MATNLYGATF PEWFGDLSKS LYTLFQVMTL ESWSMGIVRP VMNVHPNAWV FFIPFIMLTT FTVLNLFCGI IVDAMAITKE QEEEAKTGHH QEPISQTLLH LGDRLDRIEK QLAQNNELLQ RQQPQKK | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | Soduium channel | ? |
| Keyword/phrase B | Experiment 3 of 3 | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Geancarlo Zanatta, Altin Sula, Andrew J. Miles, Dave Leo Ng , Paul G. DeCaen, B.A. Wallace | ? |
| Publication Year | 2019 | ? |
| Publication Journal | Proc Natl Acad Sci (USA) | ? |
| Publication Title | Valproic Acid Interactions with the NavMs Voltage-Gated Sodium Channel | ? |
| Publication Volume | https://doi.org/10.1073/pnas.1909696116 | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2019-12-02, 11:57am. - PASS (Incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |