View & Download CD data for CD0006158000
Acetomicrobium Hydrogeniformans Esterase (ahest recombinant protein)
Citation: Kumagai PS, Gutierrez RF, Lopes JLS, Martins JM, Jameson DM, Castro AM, Martins LF, DeMarco R, Bossolan NRS, Wallace BA, Araujo APU 2018 Extremophile
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | ---- Please select ---- | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2018-08-16, 7:22pm. - FLAG
| Downloads | 1223 |
| Depositor | Jose Lopes |
| Uniprot |
|
| Alpha Fold | |
| PDB |
|
| EC | 3.1.1.x |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | Acetomicrobium hydrogeniformans esterase | ? |
| Alternative Protein Names | AhEst recombinant protein | ? |
| Source Organism | Acetomicrobium hydrogeniformans | ? |
| Protein Supplier | APU Araujo, IF-USP, Brazil | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | hexahistidineSUMO protein, cleaved | ? |
| Ligands Present and Concentration or ratio | no ligands | ? |
| Macromolecular Partner(s) and Concentration or ratio | no partner | ? |
| Deposition Date | 2018-08-17 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 0.7 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 10 mM sodium phosphate (pH 7.0) | ? |
| Baseline Contents | 10 mM sodium phosphate (pH 7.0) | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0098 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2016-11-01 | ? |
| Local Spectrum Identifier | 52298 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.4 | ? |
| CSA/ACS Pathlength (mm) | 0.099 | ? |
| CSA/ACS Zeroed at | 230-235 | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.07 | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | No data provided | ? |
| Enzyme Classification (EC) | 3.1.1.x | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | GLAGTNPLLQ RAEGAQTLKE EIITYNHEDT VLEGYLVYEE SVKDKRPGVI IIHEWMGLSE YEKMRARQIA ELGYVAFAAD IYGKGVRPKN ADEAAQLAGK FRSDRNLLRE RGQAALSVLQ NHPLVDPERI ATMGYCFGGG ASLELARSGA PIRGAISFHG NLDTPNPDDA KNIKGSILVL HGADDPMVPM EQVIAFQEEM RSANVDWQMI FYGNTVHSFT NPNAGNNPEI GVAYNPISER RSFEAMKAFF DEIFAK | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | esterase | ? |
| Keyword/phrase B | enhanced oil recovery (EOR) | ? |
| Keyword/phrase C | enzyme | ? |
| Keyword/phrase D | thermo stable protein | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Kumagai PS, Gutierrez RF, Lopes JLS, Martins JM, Jameson DM, Castro AM, Martins LF, DeMarco R, Bossolan NRS, Wallace BA, Araujo APU | ? |
| Publication Year | 2018 | ? |
| Publication Journal | Extremophile | ? |
| Publication Title | Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2018-08-16, 7:22pm. - FLAG
Depositors Notes: 1) A 27-residue signal peptide at N-terminus was removed from sequence deposited in UniProt
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | calculation failed | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | 1) A 27-residue signal peptide at N-terminus was removed from sequence deposited in UniProt | ? |