View & Download CD data for CD0006154000
Repetitive Domain Spider Dragline Silk 15-mer
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | ---- Please select ---- | File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | ---- Please select ---- | File not provided | |
| Final Processed Spectrum | Mean Residue Ellipticity | Download |
View Plot |
Validation report compiled by Validichro v1.4.0.1, 2018-04-25, 3:14am. - FLAG (Incomplete)
At a glance
| Downloads | 737 |
| Depositor | nur alia oktaviani |
| Uniprot |
P19837 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble fibrous |
Sample
| Protein Name | repetitive domain spider dragline silk 15-mer | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Nephila clavipes | ? |
| Protein Supplier | Biomacromolecules research team RIKEN CSRS Japan | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2018-04-26 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 1.6 | ? |
| Concentration Quantification Method | A280 or A280-denatured | ? |
| Protein Purity (%) | 99% | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 10 mM Phosphate buffer pH 7 | ? |
| Baseline Contents | 10 mM Phosphate buffer pH 7 | ? |
| Experimental Temperature (C) | 10 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 10 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 240 | ? |
| Minimum (lowest) wavelength, nm | 190 | ? |
| Criteria for low wavelength cutoff | No data provided | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2017-04-19 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | No data provided | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P19837 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | METHHHHHHS SGLVPRGSGM ETKETAAAKF ERQHMETDSP DLGTDDDDKA METAASGDLN NTSGRGGLGG QGAGAAAAAG GAGQGGYGGL GSQGTSGRGG LGGQGAGAAA AAGGAGQGGY GGLGSQGTSG RGGLGGQGAG AAAAAGGAGQ GGYGGLGSQG TSGRGGLGGQ GAGAAAAAGG AGQGGYGGLG SQGTSGRGGL GGQGAGAAAA AGGAGQGGYG GLGSQGTSGR GGLGGQGAGA AAAAGGAGQG GYGGLGSQGT SGRGGLGGQG AGAAAAAGGA GQGGYGGLGS QGTSGRGGLG GQGAGAAAAA GGAGQGGYGG LGSQGTSGRG GLGGQGAGAA AAAGGAGQGG YGGLGSQGTS GRGGLGGQGA GAAAAAGGAG QGGYGGLGSQ GTSGRGGLGG QGAGAAAAAG GAGQGGYGGL GSQGTSGRGG LGGQGAGAAA AAGGAGQGGY GGLGSQGTSG RGGLGGQGAG AAAAAGGAGQ GGYGGLGSQG TSGRGGLGGQ GAGAAAAAGG AGQGGYGGLG SQGTSGRGGL GGQGAGAAAA AGGAGQGGYG GLGSQGTS | ? |
| Type of protein | soluble fibrous | ? |
| Keyword/phrase A | recombinant repetitive domain | ? |
| Keyword/phrase B | spider dragline silk | ? |
| Keyword/phrase C | 15-mer | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2018-04-25, 3:14am. - FLAG (Incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | FLAG | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | INSUFFICIENT DATA | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | FLAG | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |