View & Download CD data for CD0006122000
Actin
Citation: James Tolchard, Samuel J. Walpole, Andrew J. Miles, Robin Maytum, Lawrence A. Eaglen, Ted Hackstadt, B. A. Wallace & Tharin M. A. Blumenschein 2018 Scientific Reports 8 1960
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Delta_Epsilon | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2019-11-22, 9:57am. - PASS (Incomplete)
| Downloads | 1045 |
| Depositor | Andrew Miles |
| Uniprot |
P68139 |
| Alpha Fold | |
| PDB |
1MDU |
| EC | |
| CATH Class | |
| Protein Type | membrane |
Sample
| Protein Name | Actin | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Chicken muscle | ? |
| Protein Supplier | author | ? |
| Expression System or natural source | Natural source | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2019-11-21 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 0.4 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 2mM Tris, 2mM CaCl2, 0.2 mM ATP | ? |
| Baseline Contents | 2mM Tris,mM CaCl2, 0.2 mM ATP | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2013-08-29 | ? |
| Local Spectrum Identifier | a35217 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Delta_Epsilon | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.25 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | - | ? |
| CSA/ACS Date Measured | 2013-08-28 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.07 | ? |
| CD signal at 290nm (mdeg) | 25.5 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 1MDU | ? |
| UniProt ID | P68139 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV MSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIT KQEYDEAGPS IVHRKCF | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | actin tarp complex | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | James Tolchard, Samuel J. Walpole, Andrew J. Miles, Robin Maytum, Lawrence A. Eaglen, Ted Hackstadt, B. A. Wallace & Tharin M. A. Blumenschein | ? |
| Publication Year | 2018 | ? |
| Publication Journal | Scientific Reports | ? |
| Publication Title | The intrinsically disordered Tarp protein from chlamydia binds actin with a partially preformed helix | ? |
| Publication Volume | 8 | ? |
| Publication Pages | 1960 | ? |
Validation report compiled by Validichro v1.4.0.1, 2019-11-22, 9:57am. - PASS (Incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |