View & Download CD data for CD0006121000

Translocated Actin Recruiting Phosphoprotein (tarp)

Citation: James Tolchard, Samuel J. Walpole, Andrew J. Miles, Robin M. Maytum, Lawrence A. Eaglen, Ted Hackstadt, B. A. Wallace, Tharin M. A. Blumenschein 2018 Nature, Scientific Reports Article number: 1960 8

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0.1, 2019-11-20, 3:30pm. - FLAG (Incomplete)

At a glance

Downloads	1012
Depositor	Andrew Miles
Uniprot	Q6GX35
Alpha Fold
PDB
EC
CATH Class
Protein Type	soluble globular

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Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Sample

Protein Name	translocated actin recruiting phosphoprotein	?
Alternative Protein Names	Tarp	?
Source Organism	Chlamydia trachomatis	?
Protein Supplier	author	?
Expression System or natural source	E. coli	?
Expressed As	Wild-type	?
Mutation Details	726-825	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2019-11-21	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	1.75	?
Concentration Quantification Method	Nanodrop	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	2mM Tris, 2mM CaCl2, 0.2mM ATP	?
Baseline Contents	2mM Tris, 2mM CaCl2, 0.2mM ATP	?
Experimental Temperature (C)	25	?
Instrument or beamline	ISA CD1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.00507	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Continuous	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	2	?
Experimental Collection date	2013-08-29	?
Local Spectrum Identifier	a35215	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	7.25	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	-	?
CSA/ACS Date Measured	2013-08-28	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.07	?
CD signal at 290nm (mdeg)	25.5	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	10411	?
Number of Residues	105	?
Mean Residue Weight	100	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	263 270	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

Tertiary

PDB ID	No data provided	?
UniProt ID	Q6GX35	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	GPLGSDDSGS VSSSESDKNA SVGNDGPAMK DILSAVRKHL DVVYPGDNGG STEGPLQANQ TLGDIVQDME TTGTSQETVV SPWKGSTSST GSAGGSGSVQ TLLPS	?
Type of protein	soluble globular	?
Keyword/phrase A	IDP	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	James Tolchard, Samuel J. Walpole, Andrew J. Miles, Robin M. Maytum, Lawrence A. Eaglen, Ted Hackstadt, B. A. Wallace, Tharin M. A. Blumenschein	?
Publication Year	2018	?
Publication Journal	Nature, Scientific Reports Article number: 1960	?
Publication Title	The Intrinsically Disordered Chlamydia Tarp Protein Binds Actin With A Partially Preformed Helix	?
Publication Volume	8	?
Publication Pages	No data provided	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	Dr Tharin M. A. Blumenschein	?

Validation report compiled by Validichro v1.4.0.1, 2019-11-20, 3:30pm. - FLAG (Incomplete)

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	INSUFFICIENT DATA	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	FLAG	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?