View & Download CD data for CD0005965000
Presenilin 2 (stm-2)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | ---- Please select ---- | File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | ---- Please select ---- | File not provided | |
| Final Processed Spectrum | Mean Residue Ellipticity | Download |
View Plot |
Validation report compiled by Validichro v1.4.0.1, 2017-05-25, 7:47pm. - FLAG (Incomplete)
At a glance
Sample
| Protein Name | Presenilin 2 | ? |
| Alternative Protein Names | STM-2 | ? |
| Source Organism | Human | ? |
| Protein Supplier | CSSB-FZJ,DESY | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | with tag | ? |
| Mutation Details | M1del | ? |
| Expression tags (if any) | N,MKHHHHHH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2017-05-26 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 1.06 | ? |
| Concentration Quantification Method | Extinction Coefficient | ? |
| Protein Purity (%) | 95 | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 10 mM sodium phosphate, pH 7.4, 0.014 % (w/v) FC14 | ? |
| Baseline Contents | 10 mM sodium phosphate, pH 7.4, 0.014 % (w/v) FC14 | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | Aviv | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 1 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 1 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 310 | ? |
| Minimum (lowest) wavelength, nm | 250 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 0.25 | ? |
| Dwell or Averaging time, seconds | 30 | ? |
| Experimental Collection date | 2015-04-15 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | No data provided | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P49810 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MKHHHHHHLT FMASDSEEEV CDERTSLMSA ESPTPRSCQE GRQGPEDGEN TAQWRSQENE EDGEEDPDRY VCSGVPGRPP GLEEELTLKY GAKHVIMLFV PVTLCMIVVV ATIKSVRFYT EKNGQLIYTT FTEDTPSVGQ RLLNSVLNTL IMISVIVVMT IFLVVLYKYR CYKFIHGWLI MSSLMLLFLF TYIYLGEVLK TYNVAMDYPT LLLTVWNFGA VGMVCIHWKG PLVLQQAYLI MISALMALVF IKYLPEWSAW VILGAISVYD LVAVLCPKGP LRMLVETAQE RNEPIFPALI YSSAMVWTVG MAKLDPSSQG ALQLPYDPEM EEDSYDSFGE PSYPEVFEPP LTGYPGEELE EEEERGVKLG LGDFIFYSVL VGKAAATGSG DWNTTLACFV AILIGLCLTL LLLAVFKKAL PALPISITFG LIFYFSTDNL VRPFMDTLAS HQLYI | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | Presenilin2 near UV CD | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2017-05-25, 7:47pm. - FLAG (Incomplete)
Depositors Notes:
| Missing Wavelengths | INSUFFICIENT DATA | ? |
| Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Minimal Level of Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Peak Locations | INSUFFICIENT DATA | ? |
| Feature Width | INSUFFICIENT DATA | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | INSUFFICIENT DATA | ? |
| Flat topped peaks | INSUFFICIENT DATA | ? |
| Wavelength range | INSUFFICIENT DATA | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | INSUFFICIENT DATA | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |