Formate Oxidase

Citation: No citation information currently associated with this entry.

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	---- Please select ----		File not provided
Raw Sample Spectra	---- Please select ----		File not provided
Raw Baseline Spectra	---- Please select ----		File not provided
Average Sample	---- Please select ----		File not provided
Averaged Baseline	---- Please select ----		File not provided
HT / High Voltage / Dynode Spectra	HT/Dynode Units		File not provided
Net Smoothed Spectrum	---- Please select ----		File not provided
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0.1, 2016-12-14, 5:21pm. - PASS (Incomplete)

At a glance

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Sample

Protein Name	Formate Oxidase	?
Alternative Protein Names	No data provided	?
Source Organism	none	?
Protein Supplier	Bommarius Lab	?
Expression System or natural source	E. coli	?
Expressed As	Mutant (single site)	?
Mutation Details	R87K	?
Expression tags (if any)	His-Tag	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2016-12-18	?

Experiment

CD or SRCD	CD	?
Protein Concentration (mg/ml)	0.065	?
Concentration Quantification Method	Extinction Coefficient	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	10 mM Potassium Phosphate	?
Baseline Contents	10 mM Potassium Phosphate	?
Experimental Temperature (C)	25	?
Instrument or beamline	Jasco	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.1	?
Cell Pathlength Calibration Method	Manufacturers Spec.	?
Sample Cell Type	Rectangular	?
Sample Cell Composition	Quartz	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	5	?
Continuous or Stepped scan	---- Please sele	?
Maximum (highest) wavelength, nm	300	?
Minimum (lowest) wavelength, nm	185	?
Criteria for low wavelength cutoff	No data provided	?
Wavelength interval, nm	0.5	?
Dwell or Averaging time, seconds	No data provided	?
Experimental Collection date	2015-05-27	?
Local Spectrum Identifier	No data provided	?

Calibration

CSA or ACS	No data provided	?
Dichroism Units for CSA Standard	No data provided	?
Final Spectrum Calibrated	---- Please sele	?
CSA/ACS Standard Concentration (mg/ml)	No data provided	?
CSA/ACS Pathlength (mm)	No data provided	?
CSA/ACS Zeroed at	No data provided	?
CSA/ACS Date Measured	0000-00-00	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	No data provided	?
CD signal at 290nm (mdeg)	No data provided	?
CSA/ACS Experiment temperature, C	No data provided	?

Data process.

Molecular Weight	64365	?
Number of Residues	578	?
Mean Residue Weight	111	?
Data Processing Software Name	Jasco	?
Data Processing Software Version	No data provided	?
Wavelength Range for Zeroing	No data provided	?
Number of Smoothing Points	3	?

Sec. struct.

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

Tertiary

PDB ID	3Q9T	?
UniProt ID	Q2UD26	?
Enzyme Classification (EC)	1.2.3.1	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	MATDGSHFDF VIVGGGTAGN TVAGRLAENP NVTVLIVEAG IGNPEDIPEI TTPSSAMDLR NSKYDWAYKT TMVRRDDYER IEKPNTRGKT LGGSSSLNYF TWVPGHKATF DQWEEFGGKE WTWDPLVPYL RKSATYHDDP RLYSPELEKI GGGGPIPISH AELIDEMAPF RENLTKAWKS MGQPLIENIY DGEMDGLTHC CDTIYRGQRS GSFLFVKNKP NITIVPEVHS KRLIINEADR TCKGVTVVTA AGNELNFFAD REVILSQGVF ETPKLLMLSG IGPTRELSRH GINTIVDSRH VGQNLMDHPG VPFVLRVKDG FGMDDVLLRH GPKRDAVVSA YNKNRSGPVG SGLLELVGFP RIDKYLEKDA EYRKAKAANG GKDPFSPLGQ PHFELDFVCM FPTAFQWHFP TPKTGDHLTV VVDLVRPISD PGEVTLNSAD PFQQPNINLN FFANDLDIIA MREGIRFSYD LLFKGEGFKD LVESEYPWEM PLDSDKEMHR AVLDRCQTAF HPTGTARLSK NIDQGVVDPK LKVHGIKKLR VADASVIPII PDCRIQNSVY AVGEKCADMI KAEHKDLY	?
Type of protein	soluble globular	?
Keyword/phrase A	R87K FOX	?
Keyword/phrase B	R87K FOD	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	No data provided	?
Publication Year	No data provided	?
Publication Journal	No data provided	?
Publication Title	No data provided	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor

Depositor Name	JOHN M ROBBINS	?
Department/School name	Chemical and Biomolecular Engineering	?
University/Institution/Corporation	Georgia Institute of Technology	?
Depositor Country	United States	?
Name of Principal Investigator (if not depositor)	Andreas Bommarius	?

Validation report compiled by Validichro v1.4.0.1, 2016-12-14, 5:21pm. - PASS (Incomplete)

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	INSUFFICIENT DATA	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	INSUFFICIENT DATA	?
CSA / ACS Temperature	INSUFFICIENT DATA	?
Peak Shift test	INSUFFICIENT DATA	?
Standard Deviation	INSUFFICIENT DATA	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	INSUFFICIENT DATA	?
Projection Test	PASS	?
Standard Deviation At Peak	INSUFFICIENT DATA	?
Depositor Note		?