View & Download CD data for CD0005899000
Ciona Intestinalis Betagamma-crystallin Ca2+ Form (tunicate betagamma-crystallin)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | ---- Please select ---- | Download |
File not provided |
| Average Sample | ---- Please select ---- | Download |
File not provided |
| Averaged Baseline | ---- Please select ---- | Download |
File not provided |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
File not provided |
| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0.1, 2016-09-11, 10:56pm. - PASS (Incomplete)
| Downloads | 661 |
| Depositor | Natalia Kozlyuk |
| Uniprot |
F6Q2R9 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | Ciona intestinalis betagamma-crystallin Ca2+ form | ? |
| Alternative Protein Names | tunicate betagamma-crystallin | ? |
| Source Organism | Ciona intestinalis | ? |
| Protein Supplier | Rachel Martin laboratory | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | 1 mM CaCl2 | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2016-09-11 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.075 | ? |
| Concentration Quantification Method | A280 or A280-denatured | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10 mM Tris (pH 7.1) | ? |
| Baseline Contents | 10 mM Tris (pH 7.1), 1 mM CaCl2 | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 5 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 190 | ? |
| Criteria for low wavelength cutoff | No data provided | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2016-03-03 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | No data provided | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | F6Q2R9 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | GKIILFEDVE FGGKKLELET SVSDLNVHGF NDIVSSIIVE SGTWFVFDDE GFSGPSYKLT PGKYPNPGSW GGNDDELSSV KQQ | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Tunicate beta gamma crystallin | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0.1, 2016-09-11, 10:56pm. - PASS (Incomplete)
Depositors Notes: This deposition states that the protein has 83 residues as compared to the 84 residues that the Uniport sequence. This is due to the absence of the methionine start codon which is a common post-translational modification.
| Missing Wavelengths | INSUFFICIENT DATA | ? |
| Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Minimal Level of Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Peak Locations | INSUFFICIENT DATA | ? |
| Feature Width | INSUFFICIENT DATA | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | INSUFFICIENT DATA | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | INSUFFICIENT DATA | ? |
| Flat topped peaks | INSUFFICIENT DATA | ? |
| Wavelength range | INSUFFICIENT DATA | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | INSUFFICIENT DATA | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | This deposition states that the protein has 83 residues as compared to the 84 residues that the Uniport sequence. This is due to the absence of the methionine start codon which is a common post-translational modification. | ? |