View & Download CD data for CD0005424000
EstK
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | ---- Please select ---- | File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | ---- Please select ---- | File not provided | |
| Final Processed Spectrum | Mean Residue Ellipticity | Download |
View Plot |
Validation report compiled by Validichro v1.4.0, 2016-02-25, 3:30am. - FLAG (Incomplete)
At a glance
| Downloads | 917 |
| Depositor | Daegu University |
| Uniprot |
H6VXL7 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | EstK | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Pseudomonas Mandelii | ? |
| Protein Supplier | ChangWoo Lee | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | 6His-tag | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2016-02-25 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.375 | ? |
| Concentration Quantification Method | Bradford | ? |
| Protein Purity (%) | 100 | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 20 mM TrisHCl, pH 8.0, 25 mM KCl, 0.1 mM EDTA, and 5% glycerol | ? |
| Baseline Contents | Not determined | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Vacuum | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 190 | ? |
| Criteria for low wavelength cutoff | No data provided | ? |
| Wavelength interval, nm | 0.1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2015-10-23 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | No data provided | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | H6VXL7 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MDGVEHNTQA FLDVLNAGTG KPMEQLTPIE ARAVLVGAQA SVKLTLPKAD VSEKTIQVDG QPLSLTIVRP AGVKGELPVF MFFHGGGWVL GDFPTHERLV RDLVTGSGAA AVFVNYTPSP EAHYPVAINQ AYAATKWVAE HGKEINVDGK RLAVAGNSVG GNMAAVVALM AKDKGTPAIK FQVLLWPVTD ASFETASYNQ FAEGHFLTKN MMKWFWDNYT TDAKQRNEIY ASPLRATTAQ LKGLPPALVQ TAGADVLRDE GEAYARKLDE AGVPVTSVRY NGMIHDYGLL NVVSQVPAVR SAMLQASEEL KQHLKKLEHH HHHH | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Cold-adapted | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0, 2016-02-25, 3:30am. - FLAG (Incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | FAIL | ? |
| Peak Locations | FLAG | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | INSUFFICIENT DATA | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | calculation failed | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |