View & Download CD data for CD0004692000
Hemoglobin (haemoglobin)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0, 2015-09-04, 3:39pm. - PASS (Incomplete)
At a glance
| Downloads | 1723 |
| Depositor | Marta Bolsa-Ferruz |
| Uniprot |
P69905 P68871 P69905 P02042 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | Hemoglobin | ? |
| Alternative Protein Names | Haemoglobin | ? |
| Source Organism | Human | ? |
| Protein Supplier | Sigma-Aldrich | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2015-09-08 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 3.3 | ? |
| Concentration Quantification Method | Extinction Coefficient | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 67mM Phosphate | ? |
| Baseline Contents | water and buffer | ? |
| Experimental Temperature (C) | 37 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0024 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 291 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | No data provided | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2015-01-30 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.38 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 2015-01-30 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.193 | ? |
| CD signal at 290nm (mdeg) | 24.25 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | P69905,P68871,P69905,P02042 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MVLSPADKTN VKAAWGKVGA HAGEYGAEAL ERMFLSFPTT KTYFPHFDLS HGSAQVKGHG KKVADALTNA VAHVDDMPNA LSALSDLHAH KLRVDPVNFK LLSHCLLVTL AAHLPAEFTP AVHASLDKFL ASVSTVLTSK YRMVHLTPEE KSAVTALWGK VNVDEVGGEA LGRLLVVYPW TQRFFESFGD LSTPDAVMGN PKVKAHGKKV LGAFSDGLAH LDNLKGTFAT LSELHCDKLH VDPENFRLLG NVLVCVLAHH FGKEFTPPVQ AAYQKVVAGV ANALAHKYHM VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY RMVHLTPEEK TAVNALWGKV NVDAVGGEAL GRLLVVYPWT QRFFESFGDL SSPDAVMGNP KVKAHGKKVL GAFSDGLAHL DNLKGTFSQL SELHCDKLHV DPENFRLLGN VLVCVLARNF GKEFTPQMQA AYQKVVAGVA NALAHKYH | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | hemoglobin | ? |
| Keyword/phrase B | haemoglobin | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0, 2015-09-04, 3:39pm. - PASS (Incomplete)
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |