View & Download CD data for CD0004676000

Calexcitin

Citation: P. T. Erskine, A. Fokas, C. Muriithi, H. Rehman, L. A. Yates, A. Bowyer,I. S. Findlow, R. Hagan, J. M. Werner, A. J. Miles, B. A. Wallace S. A. Wells, S. P. Wood and J. B. Cooper 2015 Acta Cryst D 71 615-631

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees \(theta\)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 5	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 6	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 4	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 5	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 6	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.2.5, 2015-03-14, 5:39pm. - PASS

At a glance

Downloads	2693
Depositor	Andrew Miles
Uniprot	O76764
Alpha Fold
PDB	2CCM
EC
CATH Class
Protein Type	soluble globular

Go to section

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Sample

Protein Name	Calexcitin	?
Alternative Protein Names	No data provided	?
Source Organism	L. pealei	?
Protein Supplier	J.B. Cooper, Royal Free Hospital, London, UK	?
Expression System or natural source	E. coli	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	none	?
Ligands Present and Concentration or ratio	none	?
Macromolecular Partner(s) and Concentration or ratio	none	?
Deposition Date	2015-03-16	?

Experiment

CD or SRCD	CD	?
Protein Concentration (mg/ml)	1.77	?
Concentration Quantification Method	Nanodrop	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	crystallisation	?
Buffer Contents and Concentrations	50mM phosphate pH 7.5, 0.1% mercaptoethanol, 1mM CaCl	?
Baseline Contents	50mM phosphate pH 7.5, 0.1% mercaptoethanol, 1mM CaCl	?
Experimental Temperature (C)	25	?
Instrument or beamline	Aviv	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.0015	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	180	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	1	?
Experimental Collection date	2012-12-23	?
Local Spectrum Identifier	alex03	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees \(theta\)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	8.4	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	235-245	?
CSA/ACS Date Measured	2012-02-22	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.14	?
CD signal at 290nm (mdeg)	30	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	22271	?
Number of Residues	191	?
Mean Residue Weight	117.2	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	263-270	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	crystal structure	?
DSSP value: alpha helix	0.584	?
DSSP value: 3-10 helix	0.037	?
DSSP value: pi helix	0.000	?
DSSP value: beta strand	0.021	?
DSSP value: beta bridge	0.010	?
DSSP value: bonded turn	0.118	?
DSSP value: bend	0.047	?
DSSP value: loop or irregular	0.183	?

Tertiary

PDB ID	2CCM	?
UniProt ID	O76764	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	MAAHQLSDFQ RNKILRVFNT FYDCNHDGVI EWDDFELAIK KICNLHSWPT DGKKHNEARA TLKLIWDGLR KYADENEDEQ VTKEEWLKMW AECVKSVEKG ESLPEWLTKY MNFMFDVNDT SGDNIIDKHE YSTVYMSYGI PKSDCDAAFD TLSDGGKTMV TREIFARLWT EYFVSNDRGA KGNHLFGTLK L	?
Type of protein	soluble globular	?
Keyword/phrase A	calcium sensor	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	P. T. Erskine, A. Fokas, C. Muriithi, H. Rehman, L. A. Yates, A. Bowyer,I. S. Findlow, R. Hagan, J. M. Werner, A. J. Miles, B. A. Wallace S. A. Wells, S. P. Wood and J. B. Cooper	?
Publication Year	2015	?
Publication Journal	Acta Cryst D	?
Publication Title	X-ray, spectroscopic and normal-mode dynamics of calexcitin: structure-function studies of a neuronal calcium-signalling protein	?
Publication Volume	71	?
Publication Pages	615-631	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	B. A. Wallace	?

Validation report compiled by Validichro v1.2.5, 2015-03-14, 5:39pm. - PASS

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	PASS	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?