View & Download CD data for CD0004574000

Aminoglycoside Adenylyltransferase (aada6)

Citation: Maria Papadovasilaki, Dominik Oberthür, Renate Gessmann, Iosifina Sarrou, Christian Betzel, Effie Scoulica, Kyriacos Petratos 2015 Biochemistry and Biophysics Reports 4 152-157

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum ---- Please select ---- File not provided
Raw Sample Spectra Millidegrees (theta) Download
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Raw Baseline Spectra ---- Please select ---- Download
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Average Sample ---- Please select ---- Download
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Averaged Baseline ---- Please select ---- Download
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HT / High Voltage / Dynode Spectra HT/Dynode Units Download
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Net Smoothed Spectrum ---- Please select ---- Download
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Final Processed Spectrum Delta Epsilon Download
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Validation report compiled by Validichro v1.3.0, 2014-12-04, 3:32pm. - PASS (Incomplete)

At a glance
Downloads 1139
Depositor Kyriacos PETRATOS
Uniprot Q9RGC2
Alpha Fold
PDB
EC 2.7.7.47
CATH Class
Protein Type soluble globular
Go to section
Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Sample

Protein Name Aminoglycoside adenylyltransferase ?
Alternative Protein Names AadA6 ?
Source Organism Pseudomonas aeruginosa Ps100 ?
Protein Supplier Structural Biology (Petratos Lab) at IMBB-FoRTH, Heraklion ?
Expression System or natural source E. coli ?
Expressed As Wild-type ?
Mutation Details No data provided ?
Expression tags (if any) No data provided ?
Ligands Present and Concentration or ratio No data provided ?
Macromolecular Partner(s) and Concentration or ratio No data provided ?
Deposition Date 2014-12-04 ?

Experiment

CD or SRCD CD ?
Protein Concentration (mg/ml) 0.2 ?
Concentration Quantification Method A280 or A280-denatured ?
Protein Purity (%) 95 ?
Purity Quantification Method SDS gels ?
Buffer Contents and Concentrations Potassium Phosphate pH 7.5, 50 mM; Na2SO4, 150 mM ?
Baseline Contents Potassium Phosphate pH 7.5, 50 mM; Na2SO4, 150 mM ?
Experimental Temperature (C) 10 ?
Instrument or beamline Jasco ?
Detector Angle (Scattering Angle) No data provided ?
Sample Cell Pathlength (cm) 0.1 ?
Cell Pathlength Calibration Method Manufacturers Spec. ?
Sample Cell Type Rectangular ?
Sample Cell Composition Quartz ?
Sample Chamber Atmosphere Nitrogen ?
Number of repeat scans 4 ?
Continuous or Stepped scan ---- Please sele ?
Maximum (highest) wavelength, nm 350 ?
Minimum (lowest) wavelength, nm 190 ?
Criteria for low wavelength cutoff No data provided ?
Wavelength interval, nm 1 ?
Dwell or Averaging time, seconds No data provided ?
Experimental Collection date 2012-07-03 ?
Local Spectrum Identifier No data provided ?

Calibration

CSA or ACS No data provided ?
Dichroism Units for CSA Standard No data provided ?
Final Spectrum Calibrated ---- Please sele ?
CSA/ACS Standard Concentration (mg/ml) No data provided ?
CSA/ACS Pathlength (mm) No data provided ?
CSA/ACS Zeroed at No data provided ?
CSA/ACS Date Measured 0000-00-00 ?
CSA/ACS Ratio (192.5nm and 290.0 nm) No data provided ?
CD signal at 290nm (mdeg) No data provided ?
CSA/ACS Experiment temperature, C No data provided ?

Data process.

Molecular Weight 30807.2 ?
Number of Residues 281 ?
Mean Residue Weight 110.0 ?
Data Processing Software Name home made ?
Data Processing Software Version No data provided ?
Wavelength Range for Zeroing No data provided ?
Number of Smoothing Points No data provided ?

Sec. struct.

Secondary Structure Calculated from No data provided ?
DSSP value: alpha helix No data provided ?
DSSP value: 3-10 helix No data provided ?
DSSP value: pi helix No data provided ?
DSSP value: beta strand No data provided ?
DSSP value: beta bridge No data provided ?
DSSP value: bonded turn No data provided ?
DSSP value: bend No data provided ?
DSSP value: loop or irregular No data provided ?

Tertiary

PDB ID No data provided ?
UniProt ID Q9RGC2 ?
Enzyme Classification (EC) 2.7.7.47 ?
Medline Entry No data provided ?
Cath Classification No data provided ?
Sequence MSNAVPAEIS VQLSLALNAI ERHLESTLLA VHLYGSALDG GLKPYSDIDL LVTVAARLDE TVRQALVVDL LEISASPGQS EALRALEVTI VVHGDVVPWR YPARRELQFG EWQRKDILAG IFEPATTDVD LAILLTKVRQ HSLALAGSAA EDFFNPVPEG DLFKALSDTL KLWNSQPDWE GDERNVVLTL SRIWYSAATG KIAPKDIVAN WAMERLPDQH KPVLLEARQA YLGQGEDCLA SRADQLAAFV HFVKHEATKL LSAMPVMSNN SFKPTPLRGA A ?
Type of protein soluble globular ?
Keyword/phrase A AadA6 ?
Keyword/phrase B Aminoglycoside adenylyltransferase ?
Keyword/phrase C Streptomycin adenylyltransferase ?
Keyword/phrase D Streptomycin 3"-O-nucleotidyltransferase ?
Keyword/phrase E ANT3" ?
Keyword/phrase F antibiotic modification ?
Keyword/phrase G antibiotic resistance ?
Keyword/phrase H Pseudomonas aeruginosa ?
Keyword/phrase I doi: 10.1016/j.bbrep.2015.09.011 ?
Keyword/phrase J No data provided ?
Publication Authors Maria Papadovasilaki, Dominik Oberthür, Renate Gessmann, Iosifina Sarrou, Christian Betzel, Effie Scoulica, Kyriacos Petratos ?
Publication Year 2015 ?
Publication Journal Biochemistry and Biophysics Reports ?
Publication Title Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa. ?
Publication Volume 4 ?
Publication Pages 152-157 ?

Depositor

Depositor Name Kyriacos PETRATOS ?
Department/School name Structural Biology ?
University/Institution/Corporation Institute of Mol. Biol. & Biotechnology- FoRTH ?
Depositor Country Greece ?
Name of Principal Investigator (if not depositor) PI ?

Validation report compiled by Validichro v1.3.0, 2014-12-04, 3:32pm. - PASS (Incomplete)

Depositors Notes: I did my best!

Missing Wavelengths PASS ?
Maximum Delta Epsilon INSUFFICIENT DATA ?
Minimal Level of Maximum Delta Epsilon INSUFFICIENT DATA ?
Peak Locations INSUFFICIENT DATA ?
Feature Width PASS ?
Experimental Temperature PASS ?
UniProt sequence INSUFFICIENT DATA ?
Molecular Weight PASS ?
Number of Residues PASS ?
Mean Residue Weight value PASS ?
Concentration and Pathlength PASS ?
CSA / ACS peak ratio INSUFFICIENT DATA ?
CSA / ACS Temperature INSUFFICIENT DATA ?
Peak Shift test INSUFFICIENT DATA ?
Standard Deviation INSUFFICIENT DATA ?
Noise: 260-270nm INSUFFICIENT DATA ?
Flat topped peaks PASS ?
Wavelength range INSUFFICIENT DATA ?
Interval resolution PASS ?
High Tension Voltage 240-260nm INSUFFICIENT DATA ?
Projection Test INSUFFICIENT DATA ?
Standard Deviation At Peak INSUFFICIENT DATA ?
Depositor Note I did my best! ?

The PCDDB is a development of the Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the schools and the authors.


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