View & Download CD data for CD0004553000
Poly-proline II Native (poly-l-proline)
Citation: Jose L.S. Lopes, Andrew J. Miles, Lee Whitmore, B.A. Wallace 2014 Protein Science 23 1765-1772
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | ---- Please select ---- | Download |
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| Raw Baseline Spectra | ---- Please select ---- | Download |
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| Average Sample | ---- Please select ---- | Download |
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| Averaged Baseline | ---- Please select ---- | Download |
File not provided |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.3.0, 2014-12-17, 4:46pm. - FLAG (Incomplete)
| Downloads | 819 |
| Depositor | Jose Lopes |
| Uniprot |
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| Alpha Fold | |
| PDB |
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| EC | |
| CATH Class | |
| Protein Type | soluble fibrous |
Sample
| Protein Name | poly-proline II native | ? |
| Alternative Protein Names | Poly-L-proline | ? |
| Source Organism | synthetic | ? |
| Protein Supplier | Sigma-Aldrich | ? |
| Expression System or natural source | homopolymer | ? |
| Expressed As | homopolymer | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | no ligands | ? |
| Macromolecular Partner(s) and Concentration or ratio | no partner | ? |
| Deposition Date | 2014-12-19 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 2.4 | ? |
| Concentration Quantification Method | Supplier value | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10 mM sodium phosphate (pH 7.0) | ? |
| Baseline Contents | 10 mM sodium phosphate (pH 7.0) | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 165 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | No data provided | ? |
| Experimental Collection date | 2013-01-14 | ? |
| Local Spectrum Identifier | a32364 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | 230-235 | ? |
| CSA/ACS Date Measured | 2013-01-14 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | No data provided | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP PPPPPPPPPP | ? |
| Type of protein | soluble fibrous | ? |
| Keyword/phrase A | Poly-L-proline (MW 1,000-10,000, Sigma-Aldrich) | ? |
| Keyword/phrase B | homopolymer | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Jose L.S. Lopes, Andrew J. Miles, Lee Whitmore, B.A. Wallace | ? |
| Publication Year | 2014 | ? |
| Publication Journal | Protein Science | ? |
| Publication Title | Distinct circular dichroism spectroscopic signatures of polyproline II and unordered secondary structures: Applications in secondary structure analyses. | ? |
| Publication Volume | 23 | ? |
| Publication Pages | 1765-1772 | ? |
Validation report compiled by Validichro v1.3.0, 2014-12-17, 4:46pm. - FLAG (Incomplete)
Depositors Notes: Poly-proline used presents molecular weight (MW) from 1,000-10,000. For the deposited file, authors have used the MW of a polypeptide that would be 100 prolines long (9729.6), number of residues as 100, MRW of 98.3, and a sequence of 100 proline residues
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | calculation failed | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | Poly-proline used presents molecular weight (MW) from 1,000-10,000. For the deposited file, authors have used the MW of a polypeptide that would be 100 prolines long (9729.6), number of residues as 100, MRW of 98.3, and a sequence of 100 proline residues | ? |