E5

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Citation: Dirk Windisch, Colin Ziegler, Stephan L. Grage, Jochen Buerck, Marcel Zeitler, Peter L. Gorkov, and Anne S. Ulrich 2015 Biophysical Journal 109 737 - 749

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Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

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CSA/ACS Standard Spectrum	---- Please select ----	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Mean Residue Ellipticity	Download	View Plot

Validation report compiled by Validichro v1.4.0, 2015-08-26, 4:13pm. - FLAG (Incomplete)

Downloads	1585
Depositor	Jochen Buerck
Uniprot	Q6XHC0
Alpha Fold
PDB
EC
CATH Class
Protein Type	membrane

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Protein Name	E5	?
Alternative Protein Names	No data provided	?
Source Organism	none	?
Protein Supplier	Institute of Biological Interfaces	?
Expression System or natural source	E. coli	?
Expressed As	Mutant (deletions)	?
Mutation Details	delF36-F44	?
Expression tags (if any)	deltaLE_Trp_Leader	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2015-08-26	?

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	1.044	?
Concentration Quantification Method	Nanodrop	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	HPLC	?
Buffer Contents and Concentrations	TCEP 10mM	?
Baseline Contents	DNPC 23,2mM, TCEP 10mM, H2O	?
Experimental Temperature (C)	37	?
Instrument or beamline	UV-CD12	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.0013	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	CaF2	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	270	?
Minimum (lowest) wavelength, nm	179	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	0.5	?
Dwell or Averaging time, seconds	1.5	?
Experimental Collection date	2012-12-06	?
Local Spectrum Identifier	No data provided	?

CSA or ACS	ACS	?
Dichroism Units for CSA Standard	No data provided	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	9.75	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	230-240 nm	?
CSA/ACS Date Measured	2012-12-04	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.051	?
CD signal at 290nm (mdeg)	30.44	?
CSA/ACS Experiment temperature, C	25	?

Molecular Weight	4182.2	?
Number of Residues	35	?
Mean Residue Weight	123.01	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	250-260 nm	?
Number of Smoothing Points	15	?

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

PDB ID	No data provided	?
UniProt ID	Q6XHC0	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	GMPNLWFLLF LGLVAAMQLL LLLFLLLFFL VYWDH	?
Type of protein	membrane	?
Keyword/phrase A	E5	?
Keyword/phrase B	oncoprotein	?
Keyword/phrase C	bovine papillomavirus	?
Keyword/phrase D	small unilamellar DNPC lipid vesicles	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	Dirk Windisch, Colin Ziegler, Stephan L. Grage, Jochen Buerck, Marcel Zeitler, Peter L. Gorkov, and Anne S. Ulrich	?
Publication Year	2015	?
Publication Journal	Biophysical Journal	?
Publication Title	Hydrophobic Mismatch Drives the Interaction of E5 with the Transmembrane Segment of PDGF Receptor	?
Publication Volume	109	?
Publication Pages	737 - 749	?

Depositor Name	Jochen Buerck	?
Department/School name	Institute of Biological Interfaces (IBG-2)	?
University/Institution/Corporation	Karlsruhe Institute of Technology	?
Depositor Country	Germany	?
Name of Principal Investigator (if not depositor)	Jochen Buerck	?

Depositors Notes: Flag for concentration/path length non-optimal ratio: we used relatively low conc. of peptide due to high background absorption induced by unsaturated lipid to get good signal-to-noise ratio below 200 nm. Flag for UniProt sequence: deletions at C terminus F36-F44

Missing Wavelengths	FAIL	?
Maximum Delta Epsilon	INSUFFICIENT DATA	?
Minimal Level of Maximum Delta Epsilon	INSUFFICIENT DATA	?
Peak Locations	INSUFFICIENT DATA	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	FLAG	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	FLAG	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	INSUFFICIENT DATA	?
Flat topped peaks	PASS	?
Wavelength range	INSUFFICIENT DATA	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	INSUFFICIENT DATA	?
Standard Deviation At Peak	PASS	?
Depositor Note	Flag for concentration/path length non-optimal ratio: we used relatively low conc. of peptide due to high background absorption induced by unsaturated lipid to get good signal-to-noise ratio below 200 nm. Flag for UniProt sequence: deletions at C terminus F36-F44	?