View & Download CD data for CD0004253000
EGFP
Citation: Viktoriia Postupalenko, Annie-Paule Sibler, Dominique Desplancq, Yves Nomine, Daniele Spehner, Patrick Schultz, Etienne Weiss, Guy Zuber 2014 Journal of Controlled Release 178 86-94
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | ---- Please select ---- | File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | ---- Please select ---- | File not provided | |
| Final Processed Spectrum | Delta Epsilon | Download |
View Plot |
Validation report compiled by Validichro v1.2.5, 2014-04-04, 3:29pm. - PASS
| Downloads | 1119 |
| Depositor | Yves Nomine |
| Uniprot |
C5MKY7 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | eGFP | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | none | ? |
| Protein Supplier | LCAMB | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Mutant (single site) | ? |
| Mutation Details | S65T | ? |
| Expression tags (if any) | C,HHHHHH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2014-04-04 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.83 | ? |
| Concentration Quantification Method | Bradford using BSA for calibration | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | Phosphate citrate 20mM, pH 4.8, NaCl 0 mM | ? |
| Baseline Contents | Phosphate citrate 20mM, pH 4.8, NaCl 0 mM | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 15 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 270 | ? |
| Minimum (lowest) wavelength, nm | 182 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 0.5 | ? |
| Dwell or Averaging time, seconds | 1.0 | ? |
| Experimental Collection date | 2013-09-05 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | C5MKY7 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MVSKGQELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYKA AATSEQKLIS EEDLNASAHH HHHH | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | pH stability of the eGFP protein | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Viktoriia Postupalenko, Annie-Paule Sibler, Dominique Desplancq, Yves Nomine, Daniele Spehner, Patrick Schultz, Etienne Weiss, Guy Zuber | ? |
| Publication Year | 2014 | ? |
| Publication Journal | Journal of Controlled Release | ? |
| Publication Title | Intracellular delivery of functionally active proteins using self-assembling pyridylthiourea-polyethylenimine | ? |
| Publication Volume | 178 | ? |
| Publication Pages | 86-94 | ? |
Validation report compiled by Validichro v1.2.5, 2014-04-04, 3:29pm. - PASS
Depositors Notes:
| Missing Wavelengths | calculation failed | ? |
| Maximum Delta Epsilon | calculation failed | ? |
| Minimal Level of Maximum Delta Epsilon | calculation failed | ? |
| Peak Locations | calculation failed | ? |
| Feature Width | calculation failed | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | calculation failed | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | calculation failed | ? |
| Flat topped peaks | calculation failed | ? |
| Wavelength range | calculation failed | ? |
| Interval resolution | calculation failed | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | calculation failed | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |