View & Download CD data for CD0004247000
Oxidized Yeast TOR1 FATC (residues 2438-2470 = Y1fatc)
Citation: L. A. M. Sommer, J. J. Janke, W. F. D. Bennett, J. Buerck, A.S. Ulrich, D. P. Tieleman, and S. A. Dames 2014 J. Phys. Chem. B 118 4817-4831
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | Download |
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| Raw Sample Spectra | ---- Please select ---- | Download |
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| Raw Baseline Spectra | ---- Please select ---- | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Mean Residue Ellipticity | Download |
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Validation report compiled by Validichro v1.3.0, 2014-05-27, 11:45am. - PASS (Incomplete)
| Downloads | 984 |
| Depositor | Jochen Buerck |
| Uniprot |
P35169 |
| Alpha Fold | |
| PDB |
2KIO
|
| EC | |
| CATH Class | |
| Protein Type | membrane |
Sample
| Protein Name | oxidized yeast TOR1 FATC (residues 2438-2470 = y1fatc) | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Saccharomyces cerevisiae | ? |
| Protein Supplier | TU Munich, Department of Chemistry | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2014-05-27 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.12 | ? |
| Concentration Quantification Method | A280 or A280-denatured | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10 mM Phosphatbuffer, 1.475mM DMPC, P:L 1:50 | ? |
| Baseline Contents | 10 mM Phosphatbuffer, 1.475mM DMPC, P:L 1:50 | ? |
| Experimental Temperature (C) | 30 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.1 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 0.1 | ? |
| Dwell or Averaging time, seconds | 8 sec Response | ? |
| Experimental Collection date | 2011-06-15 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | ACS | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 0.6 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 350 | ? |
| CSA/ACS Date Measured | 2011-04-19 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.036 | ? |
| CD signal at 290nm (mdeg) | 18.9452 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | 2KIO | ? |
| UniProt ID | P35169 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | NELDVPEQVD KLIQQATSIE RLCQHYIGWC PFW | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | ser/thr kinase | ? |
| Keyword/phrase B | in Lipidvesicles | ? |
| Keyword/phrase C | oxidized | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | L. A. M. Sommer, J. J. Janke, W. F. D. Bennett, J. Buerck, A.S. Ulrich, D. P. Tieleman, and S. A. Dames | ? |
| Publication Year | 2014 | ? |
| Publication Journal | J. Phys. Chem. B | ? |
| Publication Title | Characterization of the Immersion Properties of the Peripheral Membrane Anchor of the FATC Domain of the Kinase Target of Rapamycin by NMR, Oriented CD Spectroscopy, and MD Simulations | ? |
| Publication Volume | 118 | ? |
| Publication Pages | 4817-4831 | ? |
Validation report compiled by Validichro v1.3.0, 2014-05-27, 11:45am. - PASS (Incomplete)
Depositors Notes:
| Missing Wavelengths | INSUFFICIENT DATA | ? |
| Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Minimal Level of Maximum Delta Epsilon | INSUFFICIENT DATA | ? |
| Peak Locations | INSUFFICIENT DATA | ? |
| Feature Width | INSUFFICIENT DATA | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | INSUFFICIENT DATA | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | INSUFFICIENT DATA | ? |
| Flat topped peaks | INSUFFICIENT DATA | ? |
| Wavelength range | INSUFFICIENT DATA | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | INSUFFICIENT DATA | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |