View & Download CD data for CD0004244000
Bj-xtrIT (swiss-prot accession code p56637)
Citation: A.O. O'Reilly, A.R. Cole, J.L.S. Lopes, A. Lampert, B.A.Wallace 2014 Biochimica et Biophysica Acta 1840 10-15
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | ---- Please select ---- | Download |
File not provided |
| Averaged Baseline | ---- Please select ---- | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.2.5, 2013-10-08, 3:09pm. - FLAG
| Downloads | 898 |
| Depositor | Jose Lopes |
| Uniprot |
P56637 |
| Alpha Fold | |
| PDB |
4KYP |
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | Bj-xtrIT | ? |
| Alternative Protein Names | Swiss-Prot accession code P56637 | ? |
| Source Organism | Hottentotta judaica | ? |
| Protein Supplier | AO O'Reilly | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | His-tag | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2013-10-08 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 3.3 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 20 mM sodium phosphate; pH 7.8, 5% glycerol | ? |
| Baseline Contents | 20 mM sodium phosphate; pH 7.8, 5% glycerol | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Quartz | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 270 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2012-07-29 | ? |
| Local Spectrum Identifier | a30683 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 7.45 | ? |
| CSA/ACS Pathlength (mm) | 0.099 | ? |
| CSA/ACS Zeroed at | 230-235 | ? |
| CSA/ACS Date Measured | 2012-07-29 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.09 | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | X-ray diffraction | ? |
| DSSP value: alpha helix | 0.262 | ? |
| DSSP value: 3-10 helix | 0.000 | ? |
| DSSP value: pi helix | 0.000 | ? |
| DSSP value: beta strand | 0.179 | ? |
| DSSP value: beta bridge | 0.036 | ? |
| DSSP value: bonded turn | 0.107 | ? |
| DSSP value: bend | 0.060 | ? |
| DSSP value: loop or irregular | 0.357 | ? |
Tertiary
| PDB ID | 4KYP | ? |
| UniProt ID | P56637 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | KKNGYPLDRN GKTTECSGVN AIAPHYCNSE CTKVYYAESG YCCWGACYCF GLEDDKPIGP MKDITKKYCD VQIIPSGSHH HHHH | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | toxin | ? |
| Keyword/phrase B | scorpion | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | A.O. O'Reilly, A.R. Cole, J.L.S. Lopes, A. Lampert, B.A.Wallace | ? |
| Publication Year | 2014 | ? |
| Publication Journal | Biochimica et Biophysica Acta | ? |
| Publication Title | Chaperone-mediated native folding of a -scorpion toxin in the periplasm of Escherichia coli | ? |
| Publication Volume | 1840 | ? |
| Publication Pages | 10-15 | ? |
Validation report compiled by Validichro v1.2.5, 2013-10-08, 3:09pm. - FLAG
Depositors Notes: Uniprot flagged: This construct contains a C-terminal His6 tag.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Uniprot flagged: This construct contains a C-terminal His6 tag. | ? |