View & Download CD data for CD0004232000
TRAF Domain Of The TNF Receptor-associated Factor 4 Protein (traf4, cart1, mln62, rnf83)
Citation: Rousseau A, McEwen AG, Poussin-Courmontagne P, Rognan D, Nominé Y, Rio MC, Tomasetto C, Alpy F. 2013 PLOS Biology 11(12)
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | File not provided | |
| Raw Sample Spectra | ---- Please select ---- | File not provided | |
| Raw Baseline Spectra | ---- Please select ---- | File not provided | |
| Average Sample | ---- Please select ---- | File not provided | |
| Averaged Baseline | ---- Please select ---- | File not provided | |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | File not provided | |
| Net Smoothed Spectrum | ---- Please select ---- | File not provided | |
| Final Processed Spectrum | Delta Epsilon | Download |
View Plot |
Validation report compiled by Validichro v1.2.5, 2013-08-26, 3:12pm. - FLAG
| Downloads | 767 |
| Depositor | Yves Nomine |
| Uniprot |
Q9BUZ4 |
| Alpha Fold | |
| PDB |
|
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | TRAF domain of the TNF receptor-associated factor 4 protein | ? |
| Alternative Protein Names | TRAF4, CART1, MLN62, RNF83 | ? |
| Source Organism | Human | ? |
| Protein Supplier | IGBMC | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | C,LEHHHHHH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2013-08-26 | ? |
Experiment
| CD or SRCD | CD | ? |
| Protein Concentration (mg/ml) | 0.79 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | Ammonium bicarbonate 100 mM, PH 7.00 | ? |
| Baseline Contents | Ammonium bicarbonate 100 mM, PH 7.00 | ? |
| Experimental Temperature (C) | 25.0 | ? |
| Instrument or beamline | Jasco | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.01 | ? |
| Cell Pathlength Calibration Method | Manufacturers Spec. | ? |
| Sample Cell Type | Rectangular-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 25 | ? |
| Continuous or Stepped scan | Continuous | ? |
| Maximum (highest) wavelength, nm | 260 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 0.2 | ? |
| Dwell or Averaging time, seconds | 1 | ? |
| Experimental Collection date | 2011-09-19 | ? |
| Local Spectrum Identifier | No data provided | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | ---- Please sele | ? |
| CSA/ACS Standard Concentration (mg/ml) | No data provided | ? |
| CSA/ACS Pathlength (mm) | No data provided | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 0000-00-00 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | No data provided | ? |
| CD signal at 290nm (mdeg) | No data provided | ? |
| CSA/ACS Experiment temperature, C | No data provided | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | No data provided | ? |
| UniProt ID | Q9BUZ4 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | MALVSRQRQE LQELRRELEE LSVGSDGVLI WKIGSYGRRL QEAKAKPNLE CFSPAFYTHK YGYKLQVSAF LNGNGSGEGT HLSLYIRVLP GAFDNLLEWP FARRVTFSLL DQSDPGLAKP QHVTETFHPD PNWKNFQKPG TWRGSLDESS LGFGYPKFIS HQDIRKRNYV RDDAVFIRAA VELPRKILSL EHHHHHH | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Far UV spectrum of the TRAF domain of the wt TRAF4 protein | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Rousseau A, McEwen AG, Poussin-Courmontagne P, Rognan D, Nominé Y, Rio MC, Tomasetto C, Alpy F. | ? |
| Publication Year | 2013 | ? |
| Publication Journal | PLOS Biology | ? |
| Publication Title | TRAF4 is a novel phosphoinositide-binding protein modulating tight junctions and favoring cell migration | ? |
| Publication Volume | 11(12) | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.2.5, 2013-08-26, 3:12pm. - FLAG
Depositors Notes:
| Missing Wavelengths | calculation failed | ? |
| Maximum Delta Epsilon | calculation failed | ? |
| Minimal Level of Maximum Delta Epsilon | calculation failed | ? |
| Peak Locations | calculation failed | ? |
| Feature Width | calculation failed | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | calculation failed | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | calculation failed | ? |
| Flat topped peaks | calculation failed | ? |
| Wavelength range | calculation failed | ? |
| Interval resolution | calculation failed | ? |
| High Tension Voltage 240-260nm | INSUFFICIENT DATA | ? |
| Projection Test | calculation failed | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | ? |