View & Download CD data for CD0004116000

Pulmonary Surfactant-associated Protein B Residues 259-280 (lung surfactant protein b c-terminus residues 59-80, sp-b59-80, spbc)


Citation: No citation information currently associated with this entry.


Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)


CSA/ACS Standard Spectrum ---- Please select ---- File not provided
Raw Sample Spectra Millidegrees (theta) Download
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Raw Sample Spectra 2 Millidegrees (theta) Download
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Raw Sample Spectra 3 Millidegrees (theta) Download
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Raw Sample Spectra 4 Millidegrees (theta) Download
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Raw Baseline Spectra Millidegrees (theta) Download
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Raw Baseline Spectra 2 Millidegrees (theta) Download
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Raw Baseline Spectra 3 Millidegrees (theta) Download
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Average Sample ---- Please select ---- Download
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Averaged Baseline ---- Please select ---- Download
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HT / High Voltage / Dynode Spectra HT/Dynode Units Download
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HT / High Voltage / Dynode Spectra 2 HT/Dynode Units Download
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HT / High Voltage / Dynode Spectra 3 HT/Dynode Units Download
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HT / High Voltage / Dynode Spectra 4 HT/Dynode Units Download
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Net Smoothed Spectrum ---- Please select ---- Download
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Final Processed Spectrum Mean Residue Ellipticity Download
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Validation report compiled by Validichro v1.2.5, 2013-05-15, 7:07pm. - FLAG

At a glance
Downloads 1250
Depositor Anna Kuznetsova
Uniprot P07988
Alpha Fold
PDB
EC
CATH Class
Protein Type membrane

Sample

Protein Name Pulmonary surfactant-associated protein B residues 259-280 ?
Alternative Protein Names Lung surfactant protein B C-terminus residues 59-80, SP-B59-80, SPBC ?
Source Organism Homo sapiens ?
Protein Supplier ICBR at University of Florida ?
Expression System or natural source solid phase peptide synthesis ?
Expressed As Wild-type ?
Mutation Details No data provided ?
Expression tags (if any) No data provided ?
Ligands Present and Concentration or ratio No data provided ?
Macromolecular Partner(s) and Concentration or ratio No data provided ?
Deposition Date 2013-05-16 ?

Experiment

CD or SRCD CD ?
Protein Concentration (mg/ml) 0.101 ?
Concentration Quantification Method amino acid analysis ?
Protein Purity (%) No data provided ?
Purity Quantification Method No data provided ?
Buffer Contents and Concentrations 10mM sodium phosphate buffer, 8mM DPC detergent, pH 7.4 ?
Baseline Contents 10mM sodium phosphate buffer, 8mM DPC detergent, pH 7.4 ?
Experimental Temperature (C) 45 ?
Instrument or beamline Aviv ?
Detector Angle (Scattering Angle) No data provided ?
Sample Cell Pathlength (cm) 0.1 ?
Cell Pathlength Calibration Method Manufacturers Spec. ?
Sample Cell Type Rectangular ?
Sample Cell Composition Quartz ?
Sample Chamber Atmosphere Nitrogen ?
Number of repeat scans 60 ?
Continuous or Stepped scan ---- Please sele ?
Maximum (highest) wavelength, nm 260 ?
Minimum (lowest) wavelength, nm 180 ?
Criteria for low wavelength cutoff Noise level ?
Wavelength interval, nm 1 ?
Dwell or Averaging time, seconds No data provided ?
Experimental Collection date 2009-02-11 ?
Local Spectrum Identifier No data provided ?

Calibration

CSA or ACS No data provided ?
Dichroism Units for CSA Standard No data provided ?
Final Spectrum Calibrated ---- Please sele ?
CSA/ACS Standard Concentration (mg/ml) No data provided ?
CSA/ACS Pathlength (mm) No data provided ?
CSA/ACS Zeroed at No data provided ?
CSA/ACS Date Measured 0000-00-00 ?
CSA/ACS Ratio (192.5nm and 290.0 nm) No data provided ?
CD signal at 290nm (mdeg) No data provided ?
CSA/ACS Experiment temperature, C No data provided ?

Data process.

Molecular Weight 2531 ?
Number of Residues 22 ?
Mean Residue Weight 120.5 ?
Data Processing Software Name Matlab ?
Data Processing Software Version No data provided ?
Wavelength Range for Zeroing No data provided ?
Number of Smoothing Points No data provided ?

Sec. struct.

Secondary Structure Calculated from No data provided ?
DSSP value: alpha helix No data provided ?
DSSP value: 3-10 helix No data provided ?
DSSP value: pi helix No data provided ?
DSSP value: beta strand No data provided ?
DSSP value: beta bridge No data provided ?
DSSP value: bonded turn No data provided ?
DSSP value: bend No data provided ?
DSSP value: loop or irregular No data provided ?

Tertiary

PDB ID No data provided ?
UniProt ID P07988 ?
Enzyme Classification (EC) No data provided ?
Medline Entry No data provided ?
Cath Classification No data provided ?
Sequence DTLLGRMLPQ LVCRLVLRCS MD ?
Type of protein membrane ?
Keyword/phrase A lung surfactant protein B C-terminus ?
Keyword/phrase B No data provided ?
Keyword/phrase C No data provided ?
Keyword/phrase D No data provided ?
Keyword/phrase E No data provided ?
Keyword/phrase F No data provided ?
Keyword/phrase G No data provided ?
Keyword/phrase H No data provided ?
Keyword/phrase I No data provided ?
Keyword/phrase J No data provided ?
Publication Authors No data provided ?
Publication Year No data provided ?
Publication Journal No data provided ?
Publication Title No data provided ?
Publication Volume No data provided ?
Publication Pages No data provided ?

Depositor

Depositor Name Anna Kuznetsova ?
Department/School name Biochemistry and Molecular Biology ?
University/Institution/Corporation University of Florida ?
Depositor Country United States ?
Name of Principal Investigator (if not depositor) Joanna Long ?

Validation report compiled by Validichro v1.2.5, 2013-05-15, 7:07pm. - FLAG

Depositors Notes:

Missing Wavelengths INSUFFICIENT DATA ?
Maximum Delta Epsilon INSUFFICIENT DATA ?
Minimal Level of Maximum Delta Epsilon INSUFFICIENT DATA ?
Peak Locations INSUFFICIENT DATA ?
Feature Width INSUFFICIENT DATA ?
Experimental Temperature PASS ?
UniProt sequence FLAG ?
Molecular Weight PASS ?
Number of Residues PASS ?
Mean Residue Weight value PASS ?
Concentration and Pathlength PASS ?
CSA / ACS peak ratio INSUFFICIENT DATA ?
CSA / ACS Temperature INSUFFICIENT DATA ?
Peak Shift test INSUFFICIENT DATA ?
Standard Deviation INSUFFICIENT DATA ?
Noise: 260-270nm INSUFFICIENT DATA ?
Flat topped peaks INSUFFICIENT DATA ?
Wavelength range INSUFFICIENT DATA ?
Interval resolution PASS ?
High Tension Voltage 240-260nm INSUFFICIENT DATA ?
Projection Test INSUFFICIENT DATA ?
Standard Deviation At Peak INSUFFICIENT DATA ?
Depositor Note ?

The PCDDB is a development of the Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the schools and the authors.