MEG-14 (soluble domain of micro-exon gene 14 protein)

Citation: Lopes, J.L.S., Orcia, D., Araujo, A.P.U., DeMarco, R., Wallace, B.A. 2013 Biophysical Journal 104 2512-2520

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	---- Please select ----	Download	File not provided
Averaged Baseline	---- Please select ----	Download	File not provided
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	---- Please select ----	Download	File not provided
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.2.5, 2013-01-08, 3:09pm. - FLAG

At a glance

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Sample

Protein Name	MEG-14	?
Alternative Protein Names	Soluble domain of micro-exon gene 14 protein	?
Source Organism	Schistosoma mansoni	?
Protein Supplier	D. Orcia (DeMarco group, IFSC-USP)	?
Expression System or natural source	E. coli	?
Expressed As	Mutant (deletions)	?
Mutation Details	C terminus (signal peptide) and N terminus (transmembrane tether) were deleted	?
Expression tags (if any)	His tag	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1:100	?
Deposition Date	2014-12-09	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	0.85	?
Concentration Quantification Method	BCA method	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	sodium phosphate pH 7.0	?
Baseline Contents	sodium phosphate pH 7.0	?
Experimental Temperature (C)	25	?
Instrument or beamline	ISA CD1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.0099	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	170	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	2	?
Experimental Collection date	2012-07-04	?
Local Spectrum Identifier	No data provided	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	7.45	?
CSA/ACS Pathlength (mm)	0.099	?
CSA/ACS Zeroed at	230-235	?
CSA/ACS Date Measured	2012-07-04	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.07	?
CD signal at 290nm (mdeg)	25.1	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	8556.5	?
Number of Residues	86	?
Mean Residue Weight	100	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	No data provided	?
Wavelength Range for Zeroing	263-270	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

Tertiary

PDB ID	No data provided	?
UniProt ID	G4V6N9	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	TSANSRTHGA TSTRTHGATS TAKPAASTPP KAAATSTIKP TVTTPKAAAT STIKPTVTTK PSPAKPAASN TAKPAASTPR KPHDER	?
Type of protein	soluble fibrous	?
Keyword/phrase A	intrinsically disordered protein	?
Keyword/phrase B	micro-exon gene protein	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	Lopes, J.L.S., Orcia, D., Araujo, A.P.U., DeMarco, R., Wallace, B.A.	?
Publication Year	2013	?
Publication Journal	Biophysical Journal	?
Publication Title	Folding factors and partners for the intrinsically disordered protein micro-exon gene 14 (MEG-14).	?
Publication Volume	104	?
Publication Pages	2512-2520	?

Depositor

Depositor Name	Jose Lopes	?
Department/School name	Physics	?
University/Institution/Corporation	USP-IFSC	?
Depositor Country	Brazil	?
Name of Principal Investigator (if not depositor)	BA Wallace	?

Validation report compiled by Validichro v1.2.5, 2013-01-08, 3:09pm. - FLAG

Depositors Notes: Uniprot flagged: C and N-terminus deletions

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	FLAG	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note	Uniprot flagged: C and N-terminus deletions	?