Name: PCDDB CD0003901000
Published: 2015-03-30
License: https://pcddb.cryst.bbk.ac.uk/
Keywords: PCDDB, Protein Circular Dichroism Data Bank, circular dichroism, K3 fragment of Beta-2-microglobulin, Beta-2-microglobulin 20-41 fragment, Homo sapiens, P61769, 2E8D, soluble fibrous, K3 Amyloid, SRCD, Frank Wien, Soleil DISCO, CDTool, chemical synthesis, Wild-type

View & Download CD data for CD0003901000

K3 Fragment Of Beta-2-microglobulin (beta-2-microglobulin 20-41 fragment)

Citation: No citation information currently associated with this entry.

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 5	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 6	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 5	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 4	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 5	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 6	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0, 2015-03-30, 4:15pm. - FLAG

At a glance

Downloads	2568
Depositor	Frank Wien
Uniprot	P61769
Alpha Fold
PDB	2E8D
EC
CATH Class
Protein Type	soluble fibrous

Go to section

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Sample

Protein Name	K3 fragment of Beta-2-microglobulin	?
Alternative Protein Names	Beta-2-microglobulin 20-41 fragment	?
Source Organism	Homo sapiens	?
Protein Supplier	Yuji Goto, IPR Osaka University	?
Expression System or natural source	chemical synthesis	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2015-03-30	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	0.89	?
Concentration Quantification Method	QAA	?
Protein Purity (%)	>95%	?
Purity Quantification Method	SDS gels	?
Buffer Contents and Concentrations	6 mM HCl, pH2.3	?
Baseline Contents	dialysate	?
Experimental Temperature (C)	25	?
Instrument or beamline	Soleil DISCO	?
Detector Angle (Scattering Angle)	60	?
Sample Cell Pathlength (cm)	0.00168	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	CaF2	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	6	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	275	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	1.2	?
Experimental Collection date	2010-10-04	?
Local Spectrum Identifier	paprika11	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	6.3	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	230-245	?
CSA/ACS Date Measured	2010-04-04	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.01	?
CD signal at 290nm (mdeg)	22.5	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	2497.8	?
Number of Residues	22	?
Mean Residue Weight	118.94	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	LATEST	?
Wavelength Range for Zeroing	263-270nm	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	2E8D.PDB	?
DSSP value: alpha helix	0.0	?
DSSP value: 3-10 helix	0.0	?
DSSP value: pi helix	0.0	?
DSSP value: beta strand	0.545	?
DSSP value: beta bridge	0.0	?
DSSP value: bonded turn	0.011	?
DSSP value: bend	0.114	?
DSSP value: loop or irregular	0.33	?

Tertiary

PDB ID	2E8D	?
UniProt ID	P61769	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	SNFLNCYVSG FHPSDIEVDL LK	?
Type of protein	soluble fibrous	?
Keyword/phrase A	K3 Amyloid	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	No data provided	?
Publication Year	No data provided	?
Publication Journal	No data provided	?
Publication Title	No data provided	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor

Depositor Name	Frank Wien	?
Department/School name	Experiences	?
University/Institution/Corporation	SOLEIL Synchrotron	?
Depositor Country	France	?
Name of Principal Investigator (if not depositor)	JÃ³zsef Kardos	?

Validation report compiled by Validichro v1.4.0, 2015-03-30, 4:15pm. - FLAG

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	FLAG	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	FLAG	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?