View & Download CD data for CD0003897000
Human DUTPase (dutp pyrophosphatase)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 6 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 4 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 5 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 6 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:55pm. - FLAG
| Downloads | |
| Depositor | Frank Wien |
| Uniprot |
P33316 |
| Alpha Fold | |
| PDB |
1Q5U |
| EC | 3.6.1.23 |
| CATH Class | 2.70.40.10 |
| Protein Type | soluble globular |
Sample
| Protein Name | human dUTPase | ? |
| Alternative Protein Names | dUTP pyrophosphatase | ? |
| Source Organism | Homo sapiens | ? |
| Protein Supplier | Beta Vrtessy, Institute of Enzymology, Hung. Acad. Sci. | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | Homo sapiens. Human. Organism_taxid: 9606. Gene: hdut. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008 | ? |
| Expression tags (if any) | N,HHHHHH | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2015-04-01 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 3.3 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | >95% | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 10mM Phophate buffer pH 7.5 | ? |
| Baseline Contents | dialysate | ? |
| Experimental Temperature (C) | 20 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | 60 | ? |
| Sample Cell Pathlength (cm) | 0.0024 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 6 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 271 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1.2 | ? |
| Experimental Collection date | 2012-04-04 | ? |
| Local Spectrum Identifier | paprika7 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.3 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 230-245 | ? |
| CSA/ACS Date Measured | 2012-04-04 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 1.95 | ? |
| CD signal at 290nm (mdeg) | 23.9 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 1Q5U | ? |
| UniProt ID | P33316 | ? |
| Enzyme Classification (EC) | 3.6.1.23 | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | 2.70.40.10 | ? |
| Sequence | HHHHHHMQLR FARLSEHATA PTRGSARAAG YDLYSAYDYT IPPMEKAVVK TDIQIALPSG CYGRVAPRSG LAAKHFIDVG AGVIDEDYRG NVGVVLFNFG KEKFEVKKGD RIAQLICERI FYPEIEEVQA LDDTERGSGG FGSTGKN | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Human dutp pyrophosphatase | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:55pm. - FLAG
Depositors Notes: Uniprot sequence does not match the protein sequence used because it is a cloned protein identical to the pdb structure. It does not contain the mitochondrial signal sequence, this is the 112-252 residues fragment of the Uniprot sequence. Standard deviation was not subject to a Flag using Validichro instead this protein demonstrates a flag for max magnitude. We suggest: the spectrum under analysis is perfectly valid, but novel and be worth inclusion in the reference set.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | FLAG | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Uniprot sequence does not match the protein sequence used because it is a cloned protein identical to the pdb structure. It does not contain the mitochondrial signal sequence, this is the 112-252 residues fragment of the Uniprot sequence. Standard deviation was not subject to a Flag using Validichro instead this protein demonstrates a flag for max magnitude. We suggest: the spectrum under analysis is perfectly valid, but novel and be worth inclusion in the reference set. | ? |