Beta-2-microglobulin

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Citation: No citation information currently associated with this entry.

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Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

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CSA/ACS Standard Spectrum	---- Please select ----	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 5	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 6	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 5	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 6	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Delta Epsilon	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:41pm. - FLAG

Downloads	2402
Depositor	Frank Wien
Uniprot	P61769
Alpha Fold
PDB	2YXF
EC
CATH Class	2.60.40.10
Protein Type	soluble globular

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Protein Name	Beta-2-microglobulin	?
Alternative Protein Names	No data provided	?
Source Organism	Homo sapiens	?
Protein Supplier	Jzsef Kardos, Etvs University	?
Expression System or natural source	E. coli	?
Expressed As	Wild-type	?
Mutation Details	N,M	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2015-04-01	?

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	4.35	?
Concentration Quantification Method	QAA	?
Protein Purity (%)	>95%	?
Purity Quantification Method	SDS gels	?
Buffer Contents and Concentrations	10mM NaCl, 10mM PO4 pH7	?
Baseline Contents	dialysate	?
Experimental Temperature (C)	25	?
Instrument or beamline	Soleil DISCO	?
Detector Angle (Scattering Angle)	60	?
Sample Cell Pathlength (cm)	0.0035	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	CaF2	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	6	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	286	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	3	?
Experimental Collection date	2010-04-11	?
Local Spectrum Identifier	paprika5	?

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	No data provided	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	6.3	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	230-245	?
CSA/ACS Date Measured	2010-04-11	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.02	?
CD signal at 290nm (mdeg)	22.89	?
CSA/ACS Experiment temperature, C	25	?

Molecular Weight	11862.3	?
Number of Residues	100	?
Mean Residue Weight	119.82	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	latest	?
Wavelength Range for Zeroing	263-267	?
Number of Smoothing Points	7	?

Secondary Structure Calculated from	2YXF.pdb	?
DSSP value: alpha helix	0.0	?
DSSP value: 3-10 helix	0.03	?
DSSP value: pi helix	0.0	?
DSSP value: beta strand	0.48	?
DSSP value: beta bridge	0.02	?
DSSP value: bonded turn	0.08	?
DSSP value: bend	0.09	?
DSSP value: loop or irregular	0.30	?

PDB ID	2YXF	?
UniProt ID	P61769	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	2.60.40.10	?
Sequence	MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM	?
Type of protein	soluble globular	?
Keyword/phrase A	immune system	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	No data provided	?
Publication Year	No data provided	?
Publication Journal	No data provided	?
Publication Title	No data provided	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor Name	Frank Wien	?
Department/School name	Experiences	?
University/Institution/Corporation	SOLEIL Synchrotron	?
Depositor Country	France	?
Name of Principal Investigator (if not depositor)	József Kardos	?

Depositors Notes: -The peak location is flaged most likely because the spectrum of this rare beta sheet rich protein. After reinspection of all parameters I suggest that the spectrum under analysis is perfectly valid, but novel and worth to be included in the reference set. -The used aa sequence differs only by the first aa compared to the Uniprot sequence >sp|P61769|21-119 A Methionine has been added due to the cloning and protein protease cleavage protocol. -Standard Deviation we did not observe this flag in Validichro

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	FLAG	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	FLAG	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	FLAG	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note	-The peak location is flaged most likely because the spectrum of this rare beta sheet rich protein. After reinspection of all parameters I suggest that the spectrum under analysis is perfectly valid, but novel and worth to be included in the reference set. -The used aa sequence differs only by the first aa compared to the Uniprot sequence >sp|P61769|21-119 A Methionine has been added due to the cloning and protein protease cleavage protocol. -Standard Deviation we did not observe this flag in Validichro	?