View & Download CD data for CD0003892000
Antithrombin-III (serpin c1)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees \(theta\) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 4 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 5 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 4 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 5 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:33pm. - PASS
At a glance
| Downloads | 2526 |
| Depositor | Frank Wien |
| Uniprot |
P01008 |
| Alpha Fold | |
| PDB |
1SR5 |
| EC | |
| CATH Class | 2.30.39.10.3.30.497.10 |
| Protein Type | soluble globular |
Sample
| Protein Name | Antithrombin-III | ? |
| Alternative Protein Names | Serpin C1 | ? |
| Source Organism | Homo sapiens | ? |
| Protein Supplier | Jzsef Dob, Institute of Enzymology | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | na. | ? |
| Expression tags (if any) | na. | ? |
| Ligands Present and Concentration or ratio | na. | ? |
| Macromolecular Partner(s) and Concentration or ratio | na. | ? |
| Deposition Date | 2015-04-01 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 3.57 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | >95% | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 20mMTris-Cl, 0,1mM EDTA,10mM NaCl, 10mM PO4 pH7 | ? |
| Baseline Contents | dialysate | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | 60 | ? |
| Sample Cell Pathlength (cm) | 0.0012 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 5 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 275 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1.2 | ? |
| Experimental Collection date | 2012-09-08 | ? |
| Local Spectrum Identifier | paprika3 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees \(theta\) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.3 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 245-230 | ? |
| CSA/ACS Date Measured | 2012-09-08 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.13 | ? |
| CD signal at 290nm (mdeg) | 19.15 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | DSSP - 1sr5.pdb Chain A | ? |
| DSSP value: alpha helix | 0.238 | ? |
| DSSP value: 3-10 helix | 0.028 | ? |
| DSSP value: pi helix | 0.0 | ? |
| DSSP value: beta strand | 0.231 | ? |
| DSSP value: beta bridge | 0.007 | ? |
| DSSP value: bonded turn | 0.097 | ? |
| DSSP value: bend | 0.113 | ? |
| DSSP value: loop or irregular | 0.285 | ? |
Tertiary
| PDB ID | 1SR5 | ? |
| UniProt ID | P01008 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | 2.30.39.10.3.30.497.10 | ? |
| Sequence | HGSPVDICTA KPRDIPMNPM CIYRSPEKKA TEDEGSEQKI PEATNRRVWE LSKANSRFAT TFYQHLADSK NDNDNIFLSP LSISTAFAMT KLGACNDTLQ QLMEVFKFDT ISEKTSDQIH FFFAKLNCRL YRKANKSSKL VSANRLFGDK SLTFNETYQD ISELVYGAKL QPLDFKENAE QSRAAINKWV SNKTEGRITD VIPSEAINEL TVLVLVNTIY FKGLWKSKFS PENTRKELFY KADGESCSAS MMYQEGKFRY RRVAEGTQVL ELPFKGDDIT MVLILPKPEK SLAKVEKELT PEVLQEWLDE LEEMMLVVHM PRFRIEDGFS LKEQLQDMGL VDLFSPEKSK LPGIVAEGRD DLYVSDAFHK AFLEVNEEGS EAAASTAVVI AGRSLNPNRV TFKANRPFLV FIREVPLNTI IFMGRVANPC VK | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Hydrolase/hydrolase inhibitor | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:33pm. - PASS
Depositors Notes: To be resubmitted replacing the originally submitted antithrombin spectra.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | PASS | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | To be resubmitted replacing the originally submitted antithrombin spectra. | ? |