View & Download CD data for CD0003891000
Alpha-1-antitrypsin (alpha-1 protease inhibitor)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees \(theta\) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 4 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 5 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.3.0, 2015-01-20, 2:20pm. - PASS (Incomplete)
| Downloads | 3386 |
| Depositor | Frank Wien |
| Uniprot |
P01009 |
| Alpha Fold | |
| PDB |
1QLP |
| EC | |
| CATH Class | 2.30.39.10.3.30.497.10 |
| Protein Type | soluble globular |
Sample
| Protein Name | Alpha-1-antitrypsin | ? |
| Alternative Protein Names | Alpha-1 protease inhibitor | ? |
| Source Organism | Homo sapiens | ? |
| Protein Supplier | SIGMA | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | na. | ? |
| Expression tags (if any) | na. | ? |
| Ligands Present and Concentration or ratio | na. | ? |
| Macromolecular Partner(s) and Concentration or ratio | na. | ? |
| Deposition Date | 2015-01-29 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 17 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | 99% | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 10mM phosphate | ? |
| Baseline Contents | water and buffer | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0005 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 10 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 275 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1.2 | ? |
| Experimental Collection date | 2012-09-08 | ? |
| Local Spectrum Identifier | paprika2 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees \(theta\) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.3 | ? |
| CSA/ACS Pathlength (mm) | 0.01 | ? |
| CSA/ACS Zeroed at | n/a | ? |
| CSA/ACS Date Measured | 2012-04-04 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.03 | ? |
| CD signal at 290nm (mdeg) | 20.11 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 1QLP | ? |
| UniProt ID | P01009 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | 2.30.39.10.3.30.497.10 | ? |
| Sequence | MDPQGDAAQK TDTSHHDQDH PTFNKITPNL AEFAFSLYRQ LAHQSNSTNI FFSPVSIATA FAMLSLGTKA DTHDEILEGL NFNLTEIPEA QIHEGFQELL RTLNQPDSQL QLTTGNGLFL SEGLKLVDKF LEDVKKLYHS EAFTVNFGDT EEAKKQINDY VEKGTQGKIV DLVKELDRDT VFALVNYIFF KGKWERPFEV KDTEEEDFHV DQVTTVKVPM MKRLGMFNIQ HCKKLSSWVL LMKYLGNATA IFFLPDEGKL QHLENELTHD IITKFLENED RRSASLHLPK LSITGTYDLK SVLGQLGITK VFSNGADLSG VTEEAPLKLS KAVHKAVLTI DEKGTEAAGA MFLEAIPMSI PPEVKFNKPF VFLMIEQNTK SPLFMGKVVN PTQK | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | alpha 1 | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.3.0, 2015-01-20, 2:20pm. - PASS (Incomplete)
Depositors Notes: Uniprot P01009-1 is correct. The High Tension of 775V results in a cut-off at 170nm between 260-240nm normal behavior.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Uniprot P01009-1 is correct. The High Tension of 775V results in a cut-off at 170nm between 260-240nm normal behavior. | ? |