Alpha-1-antitrypsin (alpha-1 protease inhibitor)

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Citation: No citation information currently associated with this entry.

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Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

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CSA/ACS Standard Spectrum	---- Please select ----	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 5	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 6	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 7	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 8	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 9	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 4	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 5	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 4	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 5	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 6	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 7	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 8	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 9	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:25pm. - FLAG

Downloads	3366
Depositor	Frank Wien
Uniprot	P01009
Alpha Fold
PDB	1QLP
EC
CATH Class	2.30.39.10.3.30.497.10
Protein Type	soluble globular

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Protein Name	Alpha-1-antitrypsin	?
Alternative Protein Names	Alpha-1 protease inhibitor	?
Source Organism	Homo sapiens	?
Protein Supplier	Jzsef Dob, Institute of Enzymology	?
Expression System or natural source	E. coli	?
Expressed As	Wild-type	?
Mutation Details	cloned following the 1qlp.pdb	?
Expression tags (if any)	N,M	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2015-04-01	?

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	17	?
Concentration Quantification Method	QAA	?
Protein Purity (%)	>95%	?
Purity Quantification Method	SDS gels	?
Buffer Contents and Concentrations	20 mM Tris, 0.1 mM EDTA, 300 mM NaCl	?
Baseline Contents	dialysate	?
Experimental Temperature (C)	25	?
Instrument or beamline	Soleil DISCO	?
Detector Angle (Scattering Angle)	60	?
Sample Cell Pathlength (cm)	0.0005	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	CaF2	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	10	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	275	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	1.2	?
Experimental Collection date	2012-09-08	?
Local Spectrum Identifier	paprika1	?

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	No data provided	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	6.3	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	230-245	?
CSA/ACS Date Measured	2012-09-08	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.13	?
CD signal at 290nm (mdeg)	19.15	?
CSA/ACS Experiment temperature, C	25	?

Molecular Weight	44326.6	?
Number of Residues	394	?
Mean Residue Weight	112.8	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	latest	?
Wavelength Range for Zeroing	263-267	?
Number of Smoothing Points	7	?

Secondary Structure Calculated from	1QLP.pdb	?
DSSP value: alpha helix	0.251	?
DSSP value: 3-10 helix	0.023	?
DSSP value: pi helix	0.0	?
DSSP value: beta strand	0.279	?
DSSP value: beta bridge	0.008	?
DSSP value: bonded turn	0.102	?
DSSP value: bend	0.079	?
DSSP value: loop or irregular	0.259	?

PDB ID	1QLP	?
UniProt ID	P01009	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	2.30.39.10.3.30.497.10	?
Sequence	MDPQGDAAQK TDTSHHDQDH PTFNKITPNL AEFAFSLYRQ LAHQSNSTNI FFSPVSIATA FAMLSLGTKA DTHDEILEGL NFNLTEIPEA QIHEGFQELL RTLNQPDSQL QLTTGNGLFL SEGLKLVDKF LEDVKKLYHS EAFTVNFGDT EEAKKQINDY VEKGTQGKIV DLVKELDRDT VFALVNYIFF KGKWERPFEV KDTEEEDFHV DQVTTVKVPM MKRLGMFNIQ HCKKLSSWVL LMKYLGNATA IFFLPDEGKL QHLENELTHD IITKFLENED RRSASLHLPK LSITGTYDLK SVLGQLGITK VFSNGADLSG VTEEAPLKLS KAVHKAVLTI DEKGTEAAGA MFLEAIPMSI PPEVKFNKPF VFLMIEQNTK SPLFMGKVVN PTQK	?
Type of protein	soluble globular	?
Keyword/phrase A	Serine protease inhibitor	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	No data provided	?
Publication Year	No data provided	?
Publication Journal	No data provided	?
Publication Title	No data provided	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor Name	Frank Wien	?
Department/School name	Experiences	?
University/Institution/Corporation	SOLEIL Synchrotron	?
Depositor Country	France	?
Name of Principal Investigator (if not depositor)	József Kardos	?

Depositors Notes: Uniprot sequence does not match the protein sequence because Uniprot sequence have the signal peptide which is naturally cleaved upon processing of the protein. In addition is a cloned version with an N-terminal Met added for the expression.

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	FLAG	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note	Uniprot sequence does not match the protein sequence because Uniprot sequence have the signal peptide which is naturally cleaved upon processing of the protein. In addition is a cloned version with an N-terminal Met added for the expression.	?