View & Download CD data for CD0003889000
Antithrombin-III (antithrombin)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees \(theta\) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Average Sample | ---- Please select ---- | Download |
File not provided |
| Averaged Baseline | ---- Please select ---- | Download |
File not provided |
| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | ---- Please select ---- | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.3.0, 2015-01-20, 1:07pm. - PASS (Incomplete)
| Downloads | 1595 |
| Depositor | Frank Wien |
| Uniprot |
P01008 |
| Alpha Fold | |
| PDB |
1SR5 |
| EC | |
| CATH Class | 2.30.39 |
| Protein Type | soluble globular |
Sample
| Protein Name | Antithrombin-III | ? |
| Alternative Protein Names | Antithrombin | ? |
| Source Organism | human | ? |
| Protein Supplier | Jzsef Dob | ? |
| Expression System or natural source | human plasma | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | na. | ? |
| Expression tags (if any) | na. | ? |
| Ligands Present and Concentration or ratio | na. | ? |
| Macromolecular Partner(s) and Concentration or ratio | na. | ? |
| Deposition Date | 2015-01-29 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 5.25 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | 99 | ? |
| Purity Quantification Method | QAA | ? |
| Buffer Contents and Concentrations | 20mM Tris-Cl, 0.1mM EDTA, 100mM NaCl | ? |
| Baseline Contents | Buffer | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | 80 | ? |
| Sample Cell Pathlength (cm) | 0.0012 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 5 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 275 | ? |
| Minimum (lowest) wavelength, nm | 170 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1.2 | ? |
| Experimental Collection date | 2012-09-07 | ? |
| Local Spectrum Identifier | paprika | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees \(theta\) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.3 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 236 | ? |
| CSA/ACS Date Measured | 2012-09-07 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.05 | ? |
| CD signal at 290nm (mdeg) | 21 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 1SR5 | ? |
| UniProt ID | P01008 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | 2.30.39 | ? |
| Sequence | VDICTAKPRD IPMNPMCIYR SPEKKATEDE GSEQKIPEAT NRRVWELSKA NSRFATTFYQ HLADSKNDND NIFLSPLSIS TAFAMTKLGA CNDTLQQLME VFKFDTISEK TSDQIHFFFA KLNCRLYRKA AKSSKLVSAN RLFGDKSLTF NETYQDISEL VYGAKLQPLD FKENAEQSRA AINKWVSNKT EGRITDVIPS EAINELTVLV LVNTILFKGL WKSKFSPENT RKELFYKADG ESCSASMMYQ EGKFRYRRVA EGTQVLELPF KGDDITMVLI LPKPEKSLAK VEKELTPEVL QEWLDELEEM MLVVHMPRFR IEDGFSLKEQ LQDMGLVDLF SPEKSKLPGI VAEGRDDLYV SDAFHKAFLE VNEEGSEAAA STAVVIAGRS LNPNRVTFKA NRPFLVFIRE VPLNTIIFMG RVANPCV | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Antithrombin | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.3.0, 2015-01-20, 1:07pm. - PASS (Incomplete)
Depositors Notes: Uniprot P01008-1 gives sequence information.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | INSUFFICIENT DATA | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Uniprot P01008-1 gives sequence information. | ? |