View & Download CD data for CD0003691000

NavSp (voltage-gated sodium channel )

Citation: Emily C. McCusker, Felipe Montenegro, Shoshanna C. Isaacson, Andrew J. Miles, Natalia Alveal, Carol V. Robinson, Nelson P. Barrera, and B. A. Wallace 2012

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.2.5, 2012-08-21, 6:36pm. - PASS

At a glance

Downloads	1913
Depositor	Andrew Miles
Uniprot	Q6TMY8
Alpha Fold
PDB
EC
CATH Class
Protein Type	membrane

Go to section

Sample
Experiment
Calibration
Data Process.
Sec. Struct.
Tertiary
Depositor
Validation

Sample

Protein Name	NavSp	?
Alternative Protein Names	voltage-Gated sodium channel	?
Source Organism	S.pomeroyi	?
Protein Supplier	E.Mccusker, (Wallace group) Birkbeck college, Uni. London	?
Expression System or natural source	E. coli	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	N,GSHG	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2012-08-22	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	0.59	?
Concentration Quantification Method	Nanodrop	?
Protein Purity (%)	>95%	?
Purity Quantification Method	SDS gels	?
Buffer Contents and Concentrations	10mM Tris pH 7.5, 100mM NaCl, 0.2% decylmaltoside	?
Baseline Contents	10mM Tris pH 7.5, 100mM NaCl, 0.2% DM	?
Experimental Temperature (C)	25	?
Instrument or beamline	ISA CD1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.01	?
Cell Pathlength Calibration Method	Dilution Standards	?
Sample Cell Type	Cylindrical	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	185	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	2	?
Experimental Collection date	2008-10-25	?
Local Spectrum Identifier	a30204	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	6.7	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	235-245	?
CSA/ACS Date Measured	2008-10-25	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	2.00	?
CD signal at 290nm (mdeg)	22.2	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	29307.8	?
Number of Residues	262	?
Mean Residue Weight	112.3	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	263-270	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

Tertiary

PDB ID	No data provided	?
UniProt ID	Q6TMY8	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	GSHGMQRMQA FIDRPGFGRF ITAVIMLNAV VLGMETSPYL MERWGGLILA ADRICLGIFI VEIALKLTAQ RQRFFLNGWN LFDFVIVGIA LIPAAGGLSV LRALRILRVL RVISVAPRLR RVVEGFITAL PGMASVFLLM TIIFYIGAVI ATKLFAASFP DWFGDLGLSA YTLFQIMTLE SWSMGIVRPV MQVYPYAWLF FVPFIMITTF AVVNLLVGLI VNSMQDAHHA EDGERTDAYR DEVLARLEQI DQRLNALGET KK	?
Type of protein	membrane	?
Keyword/phrase A	Voltage-gated-sodium-channel	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	Emily C. McCusker, Felipe Montenegro, Shoshanna C. Isaacson, Andrew J. Miles, Natalia Alveal, Carol V. Robinson, Nelson P. Barrera, and B. A. Wallace	?
Publication Year	2012	?
Publication Journal	No data provided	?
Publication Title	Detergent effects on sodium channel stability: Correlation of experiment and molecular dynamics	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	B.A.Wallace	?

Validation report compiled by Validichro v1.2.5, 2012-08-21, 6:36pm. - PASS

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	PASS	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?