Beta-crystallin S (gamma-s-crystallin)

Citation: P. Evans, O.A. Bateman, C. Slingsby, B.A. Wallace Crytallin database 2007 Experimental Eye Research 84 1001-1008

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees (theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees (theta)	Download	View Plot
Average Sample	Millidegrees (theta)	Download	View Plot
Averaged Baseline	Millidegrees (theta)	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees (theta)	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.2.5, 2012-08-15, 3:57pm. - PASS

At a glance

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Sample

Protein Name	Beta-crystallin S	?
Alternative Protein Names	Gamma-S-crystallin	?
Source Organism	Bos Taurus	?
Protein Supplier	C. Slingsby	?
Expression System or natural source	Natural source	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2012-08-20	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	2.29	?
Concentration Quantification Method	QAA	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	10mM phosphate pH 7.0	?
Baseline Contents	10mM phosphate pH 7.0	?
Experimental Temperature (C)	4	?
Instrument or beamline	ISA UV1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.005	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	300	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	2	?
Experimental Collection date	2004-05-11	?
Local Spectrum Identifier	a6360	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	YES	?
CSA/ACS Standard Concentration (mg/ml)	9.71	?
CSA/ACS Pathlength (mm)	0.10	?
CSA/ACS Zeroed at	-	?
CSA/ACS Date Measured	2004-05-11	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	1.91	?
CD signal at 290nm (mdeg)	27.8	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	20796.3	?
Number of Residues	177	?
Mean Residue Weight	118.2	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	263-270	?
Number of Smoothing Points	7	?

Sec. struct.

Secondary Structure Calculated from	Crystal structure	?
DSSP value: alpha helix	0.058	?
DSSP value: 3-10 helix	0.081	?
DSSP value: pi helix	0.000	?
DSSP value: beta strand	0.395	?
DSSP value: beta bridge	0.012	?
DSSP value: bonded turn	0.047	?
DSSP value: bend	0.047	?
DSSP value: loop or irregular	0.244	?

Tertiary

PDB ID	1a7h	?
UniProt ID	P06504	?
Enzyme Classification (EC)	-	?
Medline Entry	17400211	?
Cath Classification	2.60.20.10	?
Sequence	SKAGTKITFF EDKNFQGRHY DSDCDCADFH MYLSRCNSIR VEGGTWAVYE RPNFAGYMYI LPRGEYPEYQ HWMGLNDRLS SCRAVHLSSG GQYKLQIFEK GDFNGQMHET TEDCPSIMEQ FHMREVHSCK VLEGAWIFYE LPNYRGRQYL LDKKEYRKPV DWGAASPAVQ SFRRIVE	?
Type of protein	soluble globular	?
Keyword/phrase A	No data provided	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	P. Evans, O.A. Bateman, C. Slingsby, B.A. Wallace Crytallin database	?
Publication Year	2007	?
Publication Journal	Experimental Eye Research	?
Publication Title	A reference dataset for circular dichroism spectroscopy tailored for the b-gamma-crystallin lens proteins	?
Publication Volume	84	?
Publication Pages	1001-1008	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	B.A.Wallace	?

Validation report compiled by Validichro v1.2.5, 2012-08-15, 3:57pm. - PASS

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Peak Locations	PASS	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence	PASS	?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	PASS	?
CSA / ACS Temperature	PASS	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	PASS	?
Flat topped peaks	PASS	?
Wavelength range	PASS	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	PASS	?
Projection Test	PASS	?
Standard Deviation At Peak	PASS	?
Depositor Note		?