View & Download CD data for CD0001209000
Voltage-Gated Sodium Channel Pore (spp)
Citation: Emily C. McCusker, Nazzareno DAvanzo, Colin G. Nichols, and B. A. Wallace 2011 Journal of biological chemistry 286 16386-16391
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.2.5, 2012-08-14, 1:42pm. - FLAG
| Downloads | 1554 |
| Depositor | Andrew Miles |
| Uniprot |
Q6TMY8 |
| Alpha Fold | |
| PDB |
- |
| EC | - |
| CATH Class | - |
| Protein Type | membrane |
Sample
| Protein Name | voltage-Gated sodium channel pore | ? |
| Alternative Protein Names | SPP | ? |
| Source Organism | S. pomeroyi | ? |
| Protein Supplier | E.Mccusker, (Wallace group) Birkbeck college, Uni. London | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Mutant (deletions) | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2012-08-14 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 5.00 | ? |
| Concentration Quantification Method | Nanodrop | ? |
| Protein Purity (%) | >95% | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | 10mM Tris pH 7.5, 100mM NaCl, 0.2% DM | ? |
| Baseline Contents | 10mM Tris pH 7.5, 100mM NaCl, 0.2% DM | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0009 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2010-05-16 | ? |
| Local Spectrum Identifier | a17833 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.00 | ? |
| CSA/ACS Pathlength (mm) | 0.10 | ? |
| CSA/ACS Zeroed at | 235-240 | ? |
| CSA/ACS Date Measured | 2010-05-16 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.06 | ? |
| CD signal at 290nm (mdeg) | 18.45 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | No data provided | ? |
| DSSP value: alpha helix | No data provided | ? |
| DSSP value: 3-10 helix | No data provided | ? |
| DSSP value: pi helix | No data provided | ? |
| DSSP value: beta strand | No data provided | ? |
| DSSP value: beta bridge | No data provided | ? |
| DSSP value: bonded turn | No data provided | ? |
| DSSP value: bend | No data provided | ? |
| DSSP value: loop or irregular | No data provided | ? |
Tertiary
| PDB ID | - | ? |
| UniProt ID | Q6TMY8 | ? |
| Enzyme Classification (EC) | - | ? |
| Medline Entry | 21454659 | ? |
| Cath Classification | - | ? |
| Sequence | GSHMASVFLL MTIIFYIGAV IATKLFAASF PDWFGDLGLS AYTLFQIMTL ESWSMGIVRP VMQVYPYAWL FFVPFIMITT FAVVNLLVGL IVNSMQDAHH AEDGERTDAY RDEVLARLEQ IDQRLNALGE TKK | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | voltage-gated sodium chanel | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Emily C. McCusker, Nazzareno DAvanzo, Colin G. Nichols, and B. A. Wallace | ? |
| Publication Year | 2011 | ? |
| Publication Journal | Journal of biological chemistry | ? |
| Publication Title | Simplified Bacterial Pore Channel Provides Insight into the Assembly, Stability, and Structure of Sodium Channels | ? |
| Publication Volume | 286 | ? |
| Publication Pages | 16386-16391 | ? |
Validation report compiled by Validichro v1.2.5, 2012-08-14, 1:42pm. - FLAG
Depositors Notes: Uniprot flagged because this is the Pore domain only
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Uniprot flagged because this is the Pore domain only | ? |