View & Download CD data for CD0001197000
Sodium/potassium-transporting ATPase (na,k,atpase)
Citation: Andrew J. Miles, B. A. Wallace, and Mikael Esmann 2011 BBA biomembranes 1808 2573-2580
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.2.5, 2012-02-02, 2:27pm. - PASS
At a glance
| Downloads | 1707 |
| Depositor | Andrew Miles |
| Uniprot |
Q4H132 Q70Q12 |
| Alpha Fold | |
| PDB |
2zxe |
| EC | - |
| CATH Class | 2.70.150.10 1.20.1110.10 3.40.50.1000 3.40.1110.10 |
| Protein Type | membrane |
Sample
| Protein Name | Sodium/potassium-transporting ATPase | ? |
| Alternative Protein Names | Na,K,ATPase | ? |
| Source Organism | Squalus acanthias | ? |
| Protein Supplier | M.Esmann, Uni. Aarhus, Denmark | ? |
| Expression System or natural source | Natural source | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2012-02-02 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 4.01 | ? |
| Concentration Quantification Method | Lowery (Calibrated by QAA) | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | membrane +10 mM Tris, 25 mM K2SO4 and 25% glycerol (pH 7.4) | ? |
| Baseline Contents | membrane +10 mM Tris, 25 mM K2SO4 and 25% glycerol (pH 7.4) | ? |
| Experimental Temperature (C) | 35 | ? |
| Instrument or beamline | ISA CD1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 2 | ? |
| Experimental Collection date | 2010-04-30 | ? |
| Local Spectrum Identifier | a17431 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.00 | ? |
| CSA/ACS Pathlength (mm) | 0.10 | ? |
| CSA/ACS Zeroed at | 235-240 | ? |
| CSA/ACS Date Measured | 2010-04-30 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.06 | ? |
| CD signal at 290nm (mdeg) | 20.01 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | Crystal structure | ? |
| DSSP value: alpha helix | 0.363 | ? |
| DSSP value: 3-10 helix | 0.036 | ? |
| DSSP value: pi helix | 0.004 | ? |
| DSSP value: beta strand | 0.132 | ? |
| DSSP value: beta bridge | 0.009 | ? |
| DSSP value: bonded turn | 0.102 | ? |
| DSSP value: bend | 0.081 | ? |
| DSSP value: loop or irregular | 0.273 | ? |
Tertiary
| PDB ID | 2zxe | ? |
| UniProt ID | Q4H132,Q70Q12 | ? |
| Enzyme Classification (EC) | - | ? |
| Medline Entry | 19458722 | ? |
| Cath Classification | 2.70.150.10 1.20.1110.10 3.40.50.1000 3.40.1110.10 | ? |
| Sequence | MGKGTASDKY EPAATSENAT KSKKKGKKDK IDKKRDLDEL KKEVSMDDHK LSLDELHNKY GTDLTRGLTN ARAKEILARD GPNSLTPPPT TPEWIKFCRQ LFGGFSILLW IGAILCFLAY GIQAATEDEP ANDNLYLGVV LSTVVIVTGC FSYYQEAKSS RIMDSFKNMV PQQALVIRDG EKSTINAEFV VAGDLVEVKG GDRIPADLRI ISAHGCKVDN SSLTGESEPQ TRSPEFSSEN PLETRNIAFF STNCVEGTAR GVVVYTGDRT VMGRIATLAS GLEVGRTPIA IEIEHFIHII TGVAVFLGVS FFILSLILGY SWLEAVIFLI GIIVANVPEG LLATVTVCLT LTAKRMARKN CLVKNLEAVE TLGSTSTICS DKTGTLTQNR MTVAHMWFDN QIHEADTTEN QSGAAFDKTS ATWSALSRIA ALCNRAVFQA GQDNVPILKR SVAGDASESA LLKCIELCCG SVQGMRDRNP KIVEIPFNST NKYQLSIHEN EKSSESRYLL VMKGAPERIL DRCSTILLNG AEEPLKEDMK EAFQNAYLEL GGLGERVLGF CHFALPEDKY NEGYPFDADE PNFPTTDLCF VGLMAMIDPP RAAVPDAVGK CRSAGIKVIM VTGDHPITAK AIAKGVGIIS EGNETIEDIA ARLNIPIGQV NPRDAKACVV HGSDLKDLST EVLDDILHYH TEIVFARTSP QQKLIIVEGC QRQGAIVAVT GDGVNDSPAL KKADIGVAMG ISGSDVSKQA ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLVFII GNVPLPLGTV TILCIDLGTD MVPAISLAYE QAESDIMKRQ PRNPKTDKLV NERLISMAYG QIGMIQALGG FFSYFVILAE NGFLPMDLIG KRVRWDDRWI SDVEDSFGQQ WTYEQRKIVE FTCHTSFFIS IVVVQWADLI ICKTRRNSIF QQGMKNKILI FGLFEETALA AFLSYCPGTD VALRMYPLKP SWWFCAFPYS LIIFLYDEMR RFIIRRSPGG WVEQETYYMD PEGPDNDERF TYDYYRLRVV GLIVAAVLCV IGIIILLAGK CRCKFNQNKR TRSNSGTATA QHLLQPGEAT EC | ? |
| Type of protein | membrane | ? |
| Keyword/phrase A | Thermal stability | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Andrew J. Miles, B. A. Wallace, and Mikael Esmann | ? |
| Publication Year | 2011 | ? |
| Publication Journal | BBA biomembranes | ? |
| Publication Title | Correlation of structural and functional thermal stability of the integral membrane protein Na,K-ATPase | ? |
| Publication Volume | 1808 | ? |
| Publication Pages | 2573-2580 | ? |
Validation report compiled by Validichro v1.2.5, 2012-02-02, 2:27pm. - PASS
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | PASS | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |