View & Download CD data for CD0000089000
Neuraminidase (sialidase)
Citation: Ali Abdul-Gader, Andrew John Miles and B.A. Wallace 2011 Bioinformatics 27 1630-1636
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | DRS units | Download |
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| Raw Sample Spectra | DRS units | Download |
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| Raw Baseline Spectra | DRS units | Download |
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| Average Sample | DRS units | Download |
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| Averaged Baseline | DRS units | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | DRS units | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.2.5, 2012-08-15, 3:14pm. - PASS
At a glance
| Downloads | 830 |
| Depositor | Andrew Miles |
| Uniprot |
P0C6E9 |
| Alpha Fold | P0C6E9 |
| PDB |
1kit |
| EC | 3.2.1.18 |
| CATH Class | 2.60.120.200::2.120.10.10 |
| Protein Type | soluble_globular |
Sample
| Protein Name | Neuraminidase | ? |
| Alternative Protein Names | Sialidase | ? |
| Source Organism | Vibrio cholerae | ? |
| Protein Supplier | Sigma Aldrich, UK. Cat no: N7885 | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2012-08-15 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 5.80 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | H2O | ? |
| Baseline Contents | H2O | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | Daresbury 12.1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 280 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1 | ? |
| Experimental Collection date | 2003-09-17 | ? |
| Local Spectrum Identifier | r6372 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | DRS units | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 10.30 | ? |
| CSA/ACS Pathlength (mm) | 0.10 | ? |
| CSA/ACS Zeroed at | 235-240 | ? |
| CSA/ACS Date Measured | 2003-09-17 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 2.02 | ? |
| CD signal at 290nm (mdeg) | 48612 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
| Secondary Structure Calculated from | Crystal structure | ? |
| DSSP value: alpha helix | 0.048 | ? |
| DSSP value: 3-10 helix | 0.012 | ? |
| DSSP value: pi helix | 0.000 | ? |
| DSSP value: beta strand | 0.503 | ? |
| DSSP value: beta bridge | 0.005 | ? |
| DSSP value: bonded turn | 0.125 | ? |
| DSSP value: bend | 0.110 | ? |
| DSSP value: loop or irregular | 0.197 | ? |
Tertiary
| PDB ID | 1kit | ? |
| UniProt ID | P0C6E9 | ? |
| Enzyme Classification (EC) | 3.2.1.18 | ? |
| Medline Entry | 21505036 | ? |
| Cath Classification | 2.60.120.200::2.120.10.10 | ? |
| Sequence | ALFDYNATGD TEFDSPAKQG WMQDNTNNGS GVLTNADGMP AWLVQGIGGR AQWTYSLSTN QHAQASSFGW RMTTEMKVLS GGMITNYYAN GTQRVLPIIS LDSSGNLVVE FEGQTGRTVL ATGTAATEYH KFELVFLPGS NPSASFYFDG KLIRDNIQPT ASKQNMIVWG NGSSNTDGVA AYRDIKFEIQ GDVIFRGPDR IPSIVASSVT PGVVTAFAEK RVGGGDPGAL SNTNDIITRT SRDGGITWDT ELNLTEQINV SDEFDFSDPR PIYDPSSNTV LVSYARWPTD AAQNGDRIKP WMPNGIFYSV YDVASGNWQA PIDVTDQVKE RSFQIAGWGG SELYRRNTSL NSQQDWQSNA KIRIVDGAAN QIQVADGSRK YVVTLSIDES GGLVANLNGV SAPIILQSEH AKVHSFHDYE LQYSALNHTT TLFVDGQQIT TWAGEVSQEN NIQFGNADAQ IDGRLHVQKI VLTQQGHNLV EFDAFYLAQQ TPEVEKDLEK LGWTKIKTGN TMSLYGNASV NPGPGHGITL TRQQNISGSQ NGRLIYPAIV LDRFFLNVMS IYSDDGGSNW QTGSTLPIPF RWKSSSILET LEPSEADMVE LQNGDLLLTA RLDFNQIVNG VNYSPRQQFL SKDGGITWSL LEANNANVFS NISTGTVDAS ITRFEQSDGS HFLLFTNPQG NPAGTNGRQN LGLWFSFDEG VTWKGPIQLV NGASAYSDIY QLDSENAIVI VETDNSNMRI LRMPITLLKQ KLTLSQN | ? |
| Type of protein | soluble_globular | ? |
| Keyword/phrase A | SMP180 | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Ali Abdul-Gader, Andrew John Miles and B.A. Wallace | ? |
| Publication Year | 2011 | ? |
| Publication Journal | Bioinformatics | ? |
| Publication Title | A Reference Dataset for the Analyses of Membrane Protein Secondary Structures and Transmembrane Residues using Circular Dichroism Spectroscopy | ? |
| Publication Volume | 27 | ? |
| Publication Pages | 1630-1636 | ? |
Validation report compiled by Validichro v1.2.5, 2012-08-15, 3:14pm. - PASS
Depositors Notes:
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | PASS | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | ? |