View & Download CD data for CD0000055000
Pepsinogen
Citation: Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., 2006 Bioinformatics 22 1955-1962
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | Millidegrees | Download |
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| Raw Sample Spectra 2 | Millidegrees | Download |
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| Raw Sample Spectra 3 | Millidegrees | Download |
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| Raw Baseline Spectra | Millidegrees | Download |
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| Raw Baseline Spectra 2 | Millidegrees | Download |
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| Raw Baseline Spectra 3 | Millidegrees | Download |
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| Average Sample | Millidegrees | Download |
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| Averaged Baseline | Millidegrees | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS
| Downloads | 2275 |
| Depositor | Andrew Miles |
| Uniprot |
P00791 |
| Alpha Fold | |
| PDB |
2psg |
| EC | 3.4.23.1 |
| CATH Class | 2.40.70.10 |
| Protein Type | soluble globular |
Sample
| Protein Name | Pepsinogen | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Sus scrofa | ? |
| Protein Supplier | No data provided | ? |
| Expression System or natural source | natural source | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2009-11-27 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 60.2 | ? |
| Concentration Quantification Method | A280-denatured | ? |
| Protein Purity (%) | 95 | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | water | ? |
| Baseline Contents | water | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | Daresbury 12.1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.00038 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | No data provided | ? |
| Maximum (highest) wavelength, nm | 279 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1 | ? |
| Experimental Collection date | 2004-11-10 | ? |
| Local Spectrum Identifier | r12249 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | No data provided | ? |
| CSA/ACS Standard Concentration (mg/ml) | 10.46 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | No data provided | ? |
| CSA/ACS Date Measured | 2004-11-10 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 1.96 | ? |
| CD signal at 290nm (mdeg) | 34.7 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 2psg | ? |
| UniProt ID | P00791 | ? |
| Enzyme Classification (EC) | 3.4.23.1 | ? |
| Medline Entry | 16787970 | ? |
| Cath Classification | 2.40.70.10 | ? |
| Sequence | LVKVPLVRKK SLRQNLIKNG KLKDFLKTHK HNPASKYFPE AAALIGDEPL ENYLDTEYFG TIGIGTPAQD FTVIFDTGSS NLWVPSVYCS SLACSDHNQF NPDDSSTFEA TSQELSITYG TGSMTGILGY DTVQVGGISD TNQIFGLSET EPGSFLYYAP FDGILGLAYP SISASGATPV FDNLWDQGLV SQDLFSVYLS SNDDSGSVVL LGGIDSSYYT GSLNWVPVSV EGYWQITLDS ITMDGETIAC SGGCQAIVDT GTSLLTGPTS AIANIQSDIG ASENSDGEMV ISCSSIDSLP DIVFTINGVQ YPLSPSAYIL QDDDSCTSGF EGMDVPTSSG ELWILGDVFI RQYYTVFDRA NNKVGLAPVA | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | SP175 | ? |
| Keyword/phrase B | single domain type | ? |
| Keyword/phrase C | beta | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., | ? |
| Publication Year | 2006 | ? |
| Publication Journal | Bioinformatics | ? |
| Publication Title | A reference database for circular dichroism spectroscopy covering fold and secondary structure space | ? |
| Publication Volume | 22 | ? |
| Publication Pages | 1955-1962 | ? |
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS
Depositors Notes: NULL
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Signal to noise ratio | PASS | ? |
| Deviations from zero | PASS | ? |
| High Tension Voltage readings | PASS | ? |
| Wavelength range covered (low end / high end) | PASS PASS | ? |
| Interval resolution | PASS | ? |
| Deviation between 260-270nm | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Flat topped peaks | PASS | ? |
| CSA or ACS standard used | PASS | ? |
| Width of peaks at half height | PASS | ? |
| Peak and trough locations | PASS | ? |
| Spectral Feature Width | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| Calibration data at 290nm | PASS | ? |
| Sum of DSSP fractions | PASS | ? |
| Depositor Note | ? |