Ferredoxin

Citation: Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., 2006 Bioinformatics 22 1955-1962

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees (theta)	Download	View Plot
Raw Sample Spectra	Millidegrees	Download	View Plot
Raw Sample Spectra 2	Millidegrees	Download	View Plot
Raw Sample Spectra 3	Millidegrees	Download	View Plot
Raw Baseline Spectra	Millidegrees	Download	View Plot
Raw Baseline Spectra 2	Millidegrees	Download	View Plot
Raw Baseline Spectra 3	Millidegrees	Download	View Plot
Average Sample	Millidegrees	Download	View Plot
Averaged Baseline	Millidegrees	Download	View Plot
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 2	HT/Dynode Units	Download	View Plot
HT / High Voltage / Dynode Spectra 3	HT/Dynode Units	Download	View Plot
Net Smoothed Spectrum	Millidegrees	Download	View Plot
Final Processed Spectrum	Delta Epsilon	Download	View Plot

Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS

At a glance

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Sample

Protein Name	Ferredoxin	?
Alternative Protein Names	No data provided	?
Source Organism	Clostridium acidi-urici	?
Protein Supplier	No data provided	?
Expression System or natural source	natural source	?
Expressed As	Wild-type	?
Mutation Details	No data provided	?
Expression tags (if any)	No data provided	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2009-11-26	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	2.79	?
Concentration Quantification Method	QAA	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	water	?
Baseline Contents	water	?
Experimental Temperature (C)	4	?
Instrument or beamline	Daresbury 12.1	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.0015	?
Cell Pathlength Calibration Method	Interferometry	?
Sample Cell Type	Cylindrical-Demountable	?
Sample Cell Composition	Suprasil	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	No data provided	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	175	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	1	?
Experimental Collection date	2005-10-25	?
Local Spectrum Identifier	r17308	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees (theta)	?
Final Spectrum Calibrated	No data provided	?
CSA/ACS Standard Concentration (mg/ml)	6.57	?
CSA/ACS Pathlength (mm)	0.1	?
CSA/ACS Zeroed at	235-245	?
CSA/ACS Date Measured	2005-10-25	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	1.87	?
CD signal at 290nm (mdeg)	23.8	?
CSA/ACS Experiment temperature, C	25	?

Data process.

Molecular Weight	5499	?
Number of Residues	55	?
Mean Residue Weight	101.8	?
Data Processing Software Name	CDTool	?
Data Processing Software Version	1.4	?
Wavelength Range for Zeroing	263-270	?
Number of Smoothing Points	5	?

Sec. struct.

Secondary Structure Calculated from	PDB File	?
DSSP value: alpha helix	0.073	?
DSSP value: 3-10 helix	0.055	?
DSSP value: pi helix	0.000	?
DSSP value: beta strand	0.182	?
DSSP value: beta bridge	0.000	?
DSSP value: bonded turn	0.218	?
DSSP value: bend	0.055	?
DSSP value: loop or irregular	0.418	?

Tertiary

PDB ID	2fdn	?
UniProt ID	P00198	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	16787970	?
Cath Classification	3.30.70.20	?
Sequence	AYVINEACIS CGACEPECPV NAISSGDDRY VIDADTCIDC GACAGVCPVD APVQA	?
Type of protein	soluble globular	?
Keyword/phrase A	SP175	?
Keyword/phrase B	single domain type	?
Keyword/phrase C	mixed alpha beta	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A.,	?
Publication Year	2006	?
Publication Journal	Bioinformatics	?
Publication Title	A reference database for circular dichroism spectroscopy covering fold and secondary structure space	?
Publication Volume	22	?
Publication Pages	1955-1962	?

Depositor

Depositor Name	Andrew Miles	?
Department/School name	Crystallography	?
University/Institution/Corporation	Birkbeck	?
Depositor Country	United Kingdom	?
Name of Principal Investigator (if not depositor)	B.A. Wallace	?

Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS

Depositors Notes: NULL

Maximum Delta Epsilon	PASS	?
Minimal Level of Maximum Delta Epsilon	PASS	?
Signal to noise ratio	PASS	?
Deviations from zero	PASS	?
High Tension Voltage readings	PASS	?
Wavelength range covered (low end / high end)	PASS PASS	?
Interval resolution	PASS	?
Deviation between 260-270nm	PASS	?
Mean Residue Weight value	PASS	?
Flat topped peaks	PASS	?
CSA or ACS standard used	PASS	?
Width of peaks at half height	PASS	?
Peak and trough locations	PASS	?
Spectral Feature Width	PASS	?
CSA / ACS peak ratio	PASS	?
Calibration data at 290nm	PASS	?
Sum of DSSP fractions	PASS	?
Depositor Note		?