View & Download CD data for CD0000006000
α-chymotrypsinogen
Citation: Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., 2006 Bioinformatics 22 1955-1962
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | Millidegrees (theta) | Download |
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| Raw Sample Spectra | Millidegrees | Download |
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| Raw Sample Spectra 2 | Millidegrees | Download |
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| Raw Sample Spectra 3 | Millidegrees | Download |
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| Raw Baseline Spectra | Millidegrees | Download |
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| Raw Baseline Spectra 2 | Millidegrees | Download |
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| Raw Baseline Spectra 3 | Millidegrees | Download |
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| Average Sample | Millidegrees | Download |
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| Averaged Baseline | Millidegrees | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS
| Downloads | 1271 |
| Depositor | Andrew Miles |
| Uniprot |
P00766 |
| Alpha Fold | |
| PDB |
2cga |
| EC | 3.4.21.1 |
| CATH Class | 2.40.10.10 |
| Protein Type | soluble globular |
Sample
| Protein Name | α-chymotrypsinogen | ? |
| Alternative Protein Names | No data provided | ? |
| Source Organism | Bos taurus | ? |
| Protein Supplier | No data provided | ? |
| Expression System or natural source | natural source | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2009-11-25 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 11.1 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | No data provided | ? |
| Purity Quantification Method | No data provided | ? |
| Buffer Contents and Concentrations | water | ? |
| Baseline Contents | water | ? |
| Experimental Temperature (C) | 4 | ? |
| Instrument or beamline | ISA UV1 | ? |
| Detector Angle (Scattering Angle) | No data provided | ? |
| Sample Cell Pathlength (cm) | 0.0015 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | Suprasil | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 3 | ? |
| Continuous or Stepped scan | No data provided | ? |
| Maximum (highest) wavelength, nm | 300 | ? |
| Minimum (lowest) wavelength, nm | 175 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 3 | ? |
| Experimental Collection date | 2003-10-10 | ? |
| Local Spectrum Identifier | a4696 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | Millidegrees (theta) | ? |
| Final Spectrum Calibrated | No data provided | ? |
| CSA/ACS Standard Concentration (mg/ml) | 9.9 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 235-245 | ? |
| CSA/ACS Date Measured | 2003-10-10 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 1.88 | ? |
| CD signal at 290nm (mdeg) | 29.43 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 2cga | ? |
| UniProt ID | P00766 | ? |
| Enzyme Classification (EC) | 3.4.21.1 | ? |
| Medline Entry | 16787970 | ? |
| Cath Classification | 2.40.10.10 | ? |
| Sequence | CGVPAIQPVL SGLSRIVNGE EAVPGSWPWQ VSLQDKTGFH FCGGSLINEN WVVTAAHCGV TTSDVVVAGE FDQGSSSEKI QKLKIAKVFK NSKYNSLTIN NDITLLKLST AASFSQTVSA VCLPSASDDF AAGTTCVTTG WGLTRYTNAN TPDRLQQASL PLLSNTNCKK YWGTKIKDAM ICAGASGVSS CMGDSGGPLV CKKNGAWTLV GIVSWGSSTC STSTPGVYAR VTALVNWVQQ TLAAN | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | SP175 | ? |
| Keyword/phrase B | single domain type | ? |
| Keyword/phrase C | beta | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., | ? |
| Publication Year | 2006 | ? |
| Publication Journal | Bioinformatics | ? |
| Publication Title | A reference database for circular dichroism spectroscopy covering fold and secondary structure space | ? |
| Publication Volume | 22 | ? |
| Publication Pages | 1955-1962 | ? |
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00. - PASS
Depositors Notes: NULL
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Signal to noise ratio | PASS | ? |
| Deviations from zero | PASS | ? |
| High Tension Voltage readings | PASS | ? |
| Wavelength range covered (low end / high end) | PASS PASS | ? |
| Interval resolution | PASS | ? |
| Deviation between 260-270nm | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Flat topped peaks | PASS | ? |
| CSA or ACS standard used | PASS | ? |
| Width of peaks at half height | PASS | ? |
| Peak and trough locations | PASS | ? |
| Spectral Feature Width | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| Calibration data at 290nm | PASS | ? |
| Sum of DSSP fractions | PASS | ? |
| Depositor Note | ? |