View & Compare Averaged Baseline For CD0006230000
Pyrroline-5-carboxylate Reductase 2 (pycr2, p5cr2)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
Validation report compiled by Validichro v1.4.0.1, 2021-03-22 21:30:28. - FLAG (incomplete)
Depositors Notes: Despite multiple attempts of entering correct UniProt code (Q96C36) for Pyrroline-5-carboxylate reductase 2 (Homo sapiens) here, I kept noticing error of retrieving that UniProt code for INSUFFICIENT DATA reported. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated using the pathlength 0.01 cm, my experimental CD spectra of enzyme concentration gradients revealed 4 uM was the most feasible with my limited concentration of available protein.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | Despite multiple attempts of entering correct UniProt code (Q96C36) for Pyrroline-5-carboxylate reductase 2 (Homo sapiens) here, I kept noticing error of retrieving that UniProt code for INSUFFICIENT DATA reported. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated using the pathlength 0.01 cm, my experimental CD spectra of enzyme concentration gradients revealed 4 uM was the most feasible with my limited concentration of available protein. | ? |
| Downloads | 950 |
| Depositor | Sagar M. Patel |
| Uniprot |
Q96C36 |
| Alpha Fold | |
| PDB |
6LHM |
| EC | 1.5.1.2 |
| CATH Class | |
| Protein Type | soluble_globular |
|
|
Averaged Baseline |
|
|
HT / High Voltage / Dynode Spectra |
|
|
Net Smoothed Spectrum |
|
|
Final Processed Spectrum |