View & Compare CSA/ACS Standard Spectrum For CD0003894000
Beta-2-microglobulin
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
Validation report compiled by Validichro v1.4.0, 2015-04-01, 3:41pm. - FLAG
Depositors Notes: -The peak location is flaged most likely because the spectrum of this rare beta sheet rich protein. After reinspection of all parameters I suggest that the spectrum under analysis is perfectly valid, but novel and worth to be included in the reference set. -The used aa sequence differs only by the first aa compared to the Uniprot sequence >sp|P61769|21-119 A Methionine has been added due to the cloning and protein protease cleavage protocol. -Standard Deviation we did not observe this flag in Validichro
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | FLAG | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | FLAG | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | FLAG | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | -The peak location is flaged most likely because the spectrum of this rare beta sheet rich protein. After reinspection of all parameters I suggest that the spectrum under analysis is perfectly valid, but novel and worth to be included in the reference set. -The used aa sequence differs only by the first aa compared to the Uniprot sequence >sp|P61769|21-119 A Methionine has been added due to the cloning and protein protease cleavage protocol. -Standard Deviation we did not observe this flag in Validichro | ? |
| Downloads | 2402 |
| Depositor | Frank Wien |
| Uniprot |
P61769 |
| Alpha Fold | |
| PDB |
2YXF |
| EC | |
| CATH Class | 2.60.40.10 |
| Protein Type | soluble globular |
Raw Sample Spectra
Raw Sample Spectra 2
Raw Sample Spectra 3
Raw Sample Spectra 4
Raw Sample Spectra 5
Raw Sample Spectra 6
Raw Baseline Spectra
Raw Baseline Spectra 2
Raw Baseline Spectra 3
Raw Baseline Spectra 4
Raw Baseline Spectra 5
Raw Baseline Spectra 6
Average Sample
Averaged Baseline
HT / High Voltage / Dynode Spectra
Net Smoothed Spectrum
Final Processed Spectrum