View & Download File Net Smoothed Spectrum For CD0003999005

Sodium/potassium-transporting ATPase (na,k atpase)


Citation: Andrew J. Miles, Natalya U. Fedosova, Soren V. Hoffmann, B.A. Wallace, Mikael Esmann 2013 Biochemical and Biophysical Research Communications 435 300-305


Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)


Generic a27298.gen
Date 2012-02-13T00:00:00
Experiment ATPase (pig) detergent ratio 5, - ouabain melt3
Code m35
Low Wavelength 170
High Wavelength 280
Interval (nm) 1
Cut off (nm) 170
Machine ASTRID
Dwell time (s) 2
Cell ID bbk-1
Pathlength (cm) 0.0015
Concentration (mg/ml) 4
Cut off (nm) 170
Smoothing window 7
M.R.W. (Da) 111.6
Calibration file a27274.gen
Units mdeg
Zeroed between 263 270
Baselines a27307.d01::a27307.d02::a27307.d03
Samples a27293.d01::a27293.d02::a27293.d03
Description Tris 40 mM (SO4-salt), Na2HPO4 3 mM, MgSO4, 4 mM, Glycerol 25%, pH = 7.0, detergent 5:1
Column: 1Column: 2Column: 3Column: 4Column: 5Column: 6Column: 7Column: 8
180.08.45667E-015.20250E+001.23524E+000.00000E+005.85566E-011.14584E-01
181.02.04067E+004.82153E+001.35400E+000.00000E+009.83111E-017.19089E-02
182.02.09300E+004.51703E+002.66262E+000.00000E+006.32314E-016.42512E-02
183.03.87967E+004.27403E+003.42656E+000.00000E+001.22748E+001.68405E-01
184.04.08633E+004.08167E+004.13522E+000.00000E+009.90028E-021.78625E-01
185.04.91400E+003.93507E+005.04798E+000.00000E+002.48541E-012.33770E-01
186.05.72433E+003.82213E+005.80517E+000.00000E+003.33084E-011.34974E-01
187.06.94867E+003.73760E+006.86640E+000.00000E+001.39201E-013.96681E-02
188.07.77667E+003.67410E+007.74632E+000.00000E+003.54068E-012.49933E-02
189.08.58367E+003.62660E+008.52352E+000.00000E+008.33080E-029.89680E-02
190.08.88633E+003.59010E+009.00981E+000.00000E+001.60571E-011.18131E-01
191.09.48100E+003.55930E+009.33000E+000.00000E+002.33916E-015.53795E-02
192.09.45500E+003.53220E+009.56971E+000.00000E+001.72060E-016.46237E-02
193.09.59200E+003.50650E+009.66386E+000.00000E+001.22085E-011.81169E-02
194.09.72933E+003.48060E+009.55838E+000.00000E+003.08429E-011.99125E-01
195.09.16933E+003.45080E+009.33314E+000.00000E+001.74364E-019.87567E-02
196.08.74367E+003.41760E+008.69776E+000.00000E+001.75959E-019.05097E-02
197.07.79067E+003.38147E+007.59514E+000.00000E+001.74350E-014.72323E-02
198.06.13267E+003.34480E+006.18471E+000.00000E+007.25274E-026.42201E-02
199.04.35733E+003.30810E+004.47973E+000.00000E+003.79566E-011.66352E-01
200.02.70800E+003.27240E+002.63860E+000.00000E+002.26746E-011.25722E-01
201.09.77000E-013.23720E+008.98413E-010.00000E+002.44689E-016.85582E-02
202.0-8.56667E-013.20230E+00-6.64032E-010.00000E+002.46256E-012.11868E-01
203.0-2.10100E+003.16910E+00-2.13127E+000.00000E+001.91661E-011.08127E-01
204.0-3.20800E+003.13730E+00-3.32656E+000.00000E+001.27781E-016.77561E-02
205.0-4.50533E+003.10730E+00-4.38252E+000.00000E+008.08387E-027.73348E-02
206.0-5.16967E+003.07970E+00-5.28195E+000.00000E+001.53167E-018.01388E-03
207.0-6.13467E+003.05480E+00-6.04546E+000.00000E+002.47233E-016.39809E-02
208.0-6.38200E+003.03140E+00-6.45486E+000.00000E+001.06647E-011.03988E-01
209.0-6.83733E+003.01013E+00-6.62632E+000.00000E+001.30958E-011.94107E-01
210.0-6.45500E+002.99010E+00-6.56237E+000.00000E+004.60169E-026.08660E-02
211.0-6.40867E+002.97210E+00-6.54121E+000.00000E+004.30529E-023.27855E-02
212.0-6.51067E+002.95610E+00-6.46787E+000.00000E+001.92701E-011.80616E-02
213.0-6.63000E+002.94110E+00-6.54902E+000.00000E+005.53795E-021.87419E-01
214.0-6.46367E+002.92773E+00-6.54670E+000.00000E+002.03039E-011.34974E-01
215.0-6.53133E+002.91520E+00-6.46895E+000.00000E+002.98105E-021.03783E-01
216.0-6.31833E+002.90413E+00-6.30254E+000.00000E+001.59407E-011.26771E-01
217.0-6.20133E+002.89470E+00-6.20137E+000.00000E+003.76504E-022.22847E-01
218.0-6.01800E+002.88610E+00-6.13916E+000.00000E+001.35944E-019.05551E-02
219.0-6.22800E+002.87860E+00-6.17127E+000.00000E+001.07686E-019.69032E-02
220.0-6.27467E+002.87160E+00-6.23679E+000.00000E+009.66931E-021.52441E-01
221.0-6.24800E+002.86510E+00-6.27543E+000.00000E+007.15837E-021.46281E-01
222.0-6.23333E+002.85920E+00-6.22305E+000.00000E+001.44250E-016.49838E-02
223.0-6.07867E+002.85360E+00-6.07563E+000.00000E+001.12191E-011.98780E-01
224.0-5.94200E+002.84820E+00-5.89571E+000.00000E+001.89290E-011.70378E-01
225.0-5.55167E+002.84330E+00-5.63076E+000.00000E+001.58409E-011.76889E-01
226.0-5.43200E+002.83840E+00-5.39308E+000.00000E+001.86047E-012.31777E-01
227.0-5.03300E+002.83373E+00-5.07589E+000.00000E+001.76824E-022.03400E-01
228.0-4.86400E+002.82880E+00-4.75687E+000.00000E+004.58476E-025.44324E-02
229.0-4.26900E+002.82360E+00-4.36527E+000.00000E+001.21957E-018.62670E-02
230.0-3.99567E+002.81830E+00-3.96587E+000.00000E+008.89357E-028.67384E-02
231.0-3.57267E+002.81270E+00-3.55600E+000.00000E+009.97876E-021.18418E-01
232.0-3.16167E+002.80730E+00-3.21646E+000.00000E+001.21892E-014.05408E-02
233.0-2.87367E+002.80210E+00-2.83422E+000.00000E+008.33080E-029.29241E-02
234.0-2.48833E+002.79720E+00-2.45489E+000.00000E+005.44324E-021.72345E-01
235.0-2.08900E+002.79263E+00-2.11057E+000.00000E+009.55906E-021.33690E-01
236.0-1.74533E+002.78850E+00-1.80122E+000.00000E+001.68096E-019.08772E-02
237.0-1.60867E+002.78480E+00-1.56689E+000.00000E+008.26815E-025.57215E-02
238.0-1.41333E+002.78137E+00-1.44933E+000.00000E+006.23770E-021.32999E-01
239.0-1.26767E+002.77820E+00-1.27270E+000.00000E+001.76889E-013.92626E-02
240.0-1.20067E+002.77537E+00-1.03159E+000.00000E+004.83207E-026.96770E-02
241.0-6.87667E-012.77260E+00-8.37937E-010.00000E+002.76280E-011.47894E-01
242.0-6.14667E-012.76997E+00-6.94365E-010.00000E+001.07370E-014.97483E-02
243.0-6.70000E-012.76740E+00-5.53905E-010.00000E+004.30994E-021.89795E-02
244.0-5.47667E-012.76500E+00-4.71952E-010.00000E+002.85774E-029.47523E-02
245.0-2.88333E-012.76243E+00-4.20952E-010.00000E+001.54195E-011.85539E-01
246.0-2.79333E-012.75990E+00-3.67143E-010.00000E+001.46648E-012.72000E-01
247.0-4.89667E-012.75723E+00-2.79429E-010.00000E+001.44318E-015.53795E-02
248.0-1.95000E-012.75450E+00-1.65254E-010.00000E+008.10816E-027.14470E-02
249.09.93333E-022.75163E+00-5.95238E-020.00000E+001.13796E-017.14438E-02
250.06.13333E-022.74870E+001.06984E-020.00000E+005.05239E-024.26693E-02
251.0-1.57000E-012.74577E+00-4.15397E-020.00000E+005.35226E-021.48399E-02
252.0-1.19000E-012.74270E+00-1.01365E-010.00000E+001.80616E-021.48474E-01
253.0-5.20000E-022.73973E+00-9.78730E-020.00000E+006.87071E-025.94886E-02
254.0-2.03333E-022.73650E+001.26982E-040.00000E+001.43460E-011.39285E-01
255.0-1.70000E-022.73340E+00-3.49206E-030.00000E+001.70241E-016.90040E-02
256.01.05333E-012.73010E+001.50952E-020.00000E+001.07806E-027.88937E-02
257.0-1.43000E-012.72690E+00-1.03968E-020.00000E+007.84517E-021.77248E-01
258.05.86667E-022.72380E+00-7.45556E-020.00000E+003.23213E-021.03712E-01
259.0-1.60333E-012.72060E+00-1.06016E-010.00000E+008.67807E-021.31053E-01
260.0-1.45000E-012.71760E+00-7.12540E-020.00000E+005.35226E-021.41902E-01
261.03.56667E-022.71430E+00-3.24603E-020.00000E+009.13248E-021.15020E-01
262.02.66667E-022.71097E+004.57937E-020.00000E+001.86173E-012.15613E-02
263.01.37667E-012.70790E+009.79841E-020.00000E+001.06135E-016.78102E-02
264.01.80000E-022.70497E+008.17937E-020.00000E+003.34896E-021.07045E-01
265.01.02333E-012.70210E+002.00794E-020.00000E+001.16141E-019.89680E-02
266.0-6.10000E-022.69887E+00-5.04127E-020.00000E+001.91669E-011.59383E-01
267.0-1.63000E-012.69580E+00-8.43016E-020.00000E+007.93151E-027.14438E-02
268.0-4.63333E-022.69307E+00-7.95556E-020.00000E+001.64992E-026.03379E-02
269.0-4.63333E-022.69000E+00-4.16825E-020.00000E+008.61059E-027.87951E-02
270.05.86667E-022.68707E+00-8.39683E-030.00000E+001.10831E-018.48528E-03
271.0-6.06667E-022.68400E+007.93653E-040.00000E+006.64045E-021.11251E-01
272.03.80000E-022.68107E+008.69841E-020.00000E+004.69420E-021.90397E-01
273.01.23000E-012.67800E+008.65079E-020.00000E+004.94975E-024.57262E-02
274.02.30667E-012.67527E+009.75079E-020.00000E+001.58672E-011.14590E-01
275.0-1.13000E-012.67240E+003.43492E-020.00000E+001.24566E-012.88251E-02
276.09.00000E-032.66947E+00-1.98413E-020.00000E+008.20853E-021.03876E-01
277.0-5.53333E-022.66690E+00-2.80476E-020.00000E+001.11566E-017.11618E-02
278.02.63333E-022.66410E+002.63333E-020.00000E+009.22834E-021.76126E-01
279.0-2.66667E-032.66160E+00-2.66667E-030.00000E+001.35882E-018.25873E-02
280.0-1.97333E-012.65930E+00-1.97333E-010.00000E+008.50464E-028.57321E-02

The PCDDB is a development of the Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the schools and the authors.