View & Compare Final Processed Spectrum For CD0006229000
Pyrroline-5-carboxylate Reductase 2 (pycr2, p5cr2)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
Validation report compiled by Validichro v1.4.0.1, 2021-02-24 00:37:47. - FLAG (incomplete)
Depositors Notes: I am uncertain why Peak Locations field was flagged, because the CD spectra of this single site mutant of the enzyme may be valid with its varied peak and trough values. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated, I found the optimized concentration 4 µM was feasible for CD data collection given my limited availability of concentrated protein.
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | FLAG | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | ? | |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | FLAG | ? |
| CSA / ACS peak ratio | INSUFFICIENT DATA | ? |
| CSA / ACS Temperature | INSUFFICIENT DATA | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | INSUFFICIENT DATA | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | INSUFFICIENT DATA | ? |
| Depositor Note | I am uncertain why Peak Locations field was flagged, because the CD spectra of this single site mutant of the enzyme may be valid with its varied peak and trough values. Although the recommended protein concentration provided was not within 25.0%-245% of the optimal concentration calculated, I found the optimized concentration 4 µM was feasible for CD data collection given my limited availability of concentrated protein. | ? |
| Downloads | 1358 |
| Depositor | Sagar M. Patel |
| Uniprot |
Q96C36 |
| Alpha Fold | |
| PDB |
6LHM |
| EC | 1.5.1.2 |
| CATH Class | |
| Protein Type | soluble_globular |
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Averaged Baseline |
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HT / High Voltage / Dynode Spectra |
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Net Smoothed Spectrum |
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Final Processed Spectrum |