View & Compare CSA/ACS Standard Spectrum For CD0004265000

E5


Citation: Dirk Windisch, Colin Ziegler, Stephan L. Grage, Jochen Buerck, Marcel Zeitler, Peter L. Gorkov, and Anne S. Ulrich 2015 Biophysical Journal 109 737 - 749


Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)


Validation report compiled by Validichro v1.4.0, 2015-08-26, 4:10pm. - FLAG (Incomplete)

Depositors Notes: Flag for concentration/path length non-optimal ratio: we used relatively low conc. of peptide due to high background absorption induced by unsaturated lipid to get good signal-to-noise ratio below 200 nm. Flag for UniProt sequence: deletions at C terminus F36-F44

Missing Wavelengths PASS ?
Maximum Delta Epsilon INSUFFICIENT DATA ?
Minimal Level of Maximum Delta Epsilon INSUFFICIENT DATA ?
Peak Locations INSUFFICIENT DATA ?
Feature Width PASS ?
Experimental Temperature PASS ?
UniProt sequence FLAG ?
Molecular Weight PASS ?
Number of Residues PASS ?
Mean Residue Weight value PASS ?
Concentration and Pathlength FLAG ?
CSA / ACS peak ratio PASS ?
CSA / ACS Temperature PASS ?
Peak Shift test PASS ?
Standard Deviation PASS ?
Noise: 260-270nm INSUFFICIENT DATA ?
Flat topped peaks PASS ?
Wavelength range INSUFFICIENT DATA ?
Interval resolution PASS ?
High Tension Voltage 240-260nm PASS ?
Projection Test INSUFFICIENT DATA ?
Standard Deviation At Peak PASS ?
Depositor Note Flag for concentration/path length non-optimal ratio: we used relatively low conc. of peptide due to high background absorption induced by unsaturated lipid to get good signal-to-noise ratio below 200 nm. Flag for UniProt sequence: deletions at C terminus F36-F44 ?

The PCDDB is a development of the Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the schools and the authors.